当归提取液对培养的成年人视网膜神经细胞活性的影响  

Effect of extract of Angelica sinensis(Oliv.) Diels on cultured adult human retinal nerve cells

作　　者：沈志军[1] 王津津[1] 李根林[1] 

机构地区：[1]首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科学与视觉科学重点实验室,100730

出　　处：《眼科》2013年第6期405-408,共4页Ophthalmology in China

摘　　要：目的研究当归提取液对体外培养的成年人视网膜神经细胞活性的影响。设计实验研究。研究对象成年人视网膜神经细胞。方法体外培养成年人视网膜神经细胞,加入倍比稀释浓度(1.25、2.50、5.00、10.00、20.00、40.00、80.00 g/L)的当归提取液各50μl,培养至24 h和72 h时,以MTT法检测细胞存活和生长情况;共聚焦显微镜测定细胞线粒体膜电位荧光强度值变化,记录培养液未加入当归提取液以及两次加入当归提取液(两次加药相隔94秒)后细胞线粒体膜电位荧光强度的变化,比较首次加药、再次加药及未加药间细胞荧光强度峰值。主要指标细胞存活和生长情况,细胞线粒体膜电位峰值。结果成年人视网膜神经细胞培养至24 h及72 h时MTT法检测显示,80.00 g/L当归提取液对成年人视网膜神经细胞保护作用分别相当于对照组的256%和261%。线粒体膜电位检测荧光强度的变化显示,首次加入当归提取液后荧光强度峰值(26.02±7.62)较加药前(4.95±0.02)增加了437%(P<0.001);再次加入当归提取液后荧光强度峰值(54.17±14.15)较加药前(4.95±0.02)增加了997%(P<0.001)。表明当归提取液能够显著升高成年人视网膜神经细胞的膜电位并使其兴奋性呈波动性变化。结论当归提取液对成年人视网膜神经细胞有一定的保护作用,有望成为一种新的防治视网膜疾病的辅助治疗药物。(眼科,2013,22:405-408)Objective To study the effects of extract of Angelica sinensis （Oliv.） Diels on the cultured human retinal nerve cells. Design Experimental study. Participants Adult retinal nerve cells. Methods Retinal nerve cells were co-cultured with different con- centration of extract of Angelica sinensis （Oliv.） Diels （1.25, 2.50, 5.00, 10.00, 20.00, 40.00 and 80.00 g/L, respectively）; then 24 hours and 72 hours later, retinal nerve cells proliferation was evaluated by MTT assays; the mitochondrial membrane potentials （MMPs） of cells was measured by eonfocal microscopy. The change of fluorescence intensity before and after twice adding the extract were recorded （the interval of the twice adding was 94 seconds）. The peaks of fluorescence intensity which represented MMPs between the twice adding and base line were analysed using one-way analysis of variance. Main Outcome Measures Cell proliferation, MMPs. Results Compared to the control group, the protective effects of the extract with the highest concentration of 80.00 g/L was 256% after 24 hours and 261% after 72 hours, respectively. Confoeal microscopy showed the peaks of fluorescence intensity were significantly different a- mong first adding, second adding and the base line （F=56.694, P〈0.001）. Compared with the base line （4.95-＋0.02）, the peak of fluores- cence intensity increased 437% after the first adding of the extract （26.02±7.62, P〈0.001） and 997% after the second adding of the ex- tract （54.17±14.15, P〈0.001）. It showed that the extract could enhance the MMPs significantly with undulatory property. Conclusion The extract of Angelica sinensis （Oliv.） Diels has certain protective effects on adult retinal nerve cells and might be used in the treat- ment of some retinal diseases in the future.

关 键 词：视网膜 神经细胞 当归提取液 线粒体膜电位 

分 类 号：R285.5[医药卫生—中药学]