肠道病毒71型细胞感染灭活验证方法的建立  

A Cell Culture Method for Validation Test on Inactivation of Enterovirus 71

作　　者：刘杰[1,2] 潘海龙[2] 孙艳[2] 牟建超[2] 陈平[2] 刘辉[2] 何敏[2] 曾献武[2] 葛永红[2] 杨莉[1] 

机构地区：[1]四川大学生物治疗国家重点实验室,四川成都610041 [2]成都生物制品研究所有限责任公司,四川成都610023

出　　处：《预防医学情报杂志》2013年第11期914-917,共4页Journal of Preventive Medicine Information

摘　　要：目的建立肠道病毒71型细胞感染灭活验证的方法,为肠道病毒71型灭活疫苗的安全性提供技术保障。方法将不同浓度的肠道病毒71型接种于五种不同细胞选择敏感性好的细胞,然后将制备的8批肠道病毒71型灭活样品接种在筛选到的敏感细胞上,35℃,5%CO2连续培养21 d,观察细胞病变情况。结果 Vero细胞感染法最低能检测到1CCID50/ml的肠道病毒71型,该方法检测8批肠道病毒71型灭活样品均为阴性。结论本研究建立的肠道病毒71型细胞感染灭活验证方法具有良好的敏感性和重复性,可用于肠道病毒71型疫苗的灭活验证。Objective To develop a cell infection method for validation of inactivation of enterovirus 71 （ EV71 ） and provide a technical support for the safety of inactivated EV71 vaccine. Methods EV71 at various concen- trations were inoculated into five different cell monolayers, of which most sensitive cell were selected; after that, eight batches of inactivated EV71 samples were cultured in VERO ceils for 21d at 35 ~C and 5% CO2, finally cytopathic effect was observed. Results Vero cell infection method reached a sensitivity of 1 CCID50/ ml; the determination results of eight batches of inactivated EV71 samples by the developed cell infection meth- od were negative. Conclusion The method is of high sensitivity and reproducibility, and thus is suitable for the validation of inactivation of EV71 vaccine.

关 键 词：肠道病毒71型 细胞感染灭活验证Vero细胞 

分 类 号：R373.2[医药卫生—病原生物学]