半边旗提取物5F对乳癌细胞MDA-MB-231培养物诱导的HUVEC血管生成潜能的影响  

Effect of Pteris semipinnata L extract 5F on angiogenic abilities of HUVEC induced by the culture of MDA-MB-231 cells

作　　者：何振辉[1] 翁闪凡[1] 何太平[2] 覃燕梅[2] 梁念慈 

机构地区：[1]佛山科学技术学院医学院医学检验系,佛山528000 [2]广东医学院生物化学与分子生物学研究所,湛江524023 [3]广东省天然药物研究与开发重点实验室,湛江524023

出　　处：《郑州大学学报（医学版）》2013年第6期724-728,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目39870900;广东省中医药局建设中医药强省课题20111057;佛山市医学类科技攻关项目201108064;佛山科学技术学院博士启动基金2012006(佛山科学技术学院校级科研项目资助)

摘　　要：目的:研究半边旗提取物5F对乳癌细胞MDA-MB-231培养物(乳癌细胞培养物)诱导的人脐静脉内皮细胞(HUVEC)血管生成潜能的影响及其作用机制。方法:MTT法检测不同浓度5F对乳癌细胞培养物诱导的HUVEC增殖的抑制作用;Transwell小室法检测不同浓度5F对乳癌细胞培养物诱导的HUVEC穿膜能力和趋化性运动能力的影响;细胞-基质黏附实验检测5F对乳癌细胞培养物诱导的HUVEC黏附能力的影响;RT-PCR、Western blot法检测不同浓度5F对乳癌细胞培养物诱导的HUVEC KDR、Flt-1 mRNA和蛋白表达的影响。结果:不同浓度5F处理6、24 h后对乳癌细胞培养物诱导的HUVEC的增殖有一定程度的抑制作用(F组间=64.852,F时间=131.393,P<0.001)。20、40、80μmol/L 5F可抑制乳癌细胞培养物诱导的HUVEC体外穿膜能力、趋化性运动能力和黏附基质能力(F=48.206、147.613、22.081,P均<0.001)。不同浓度5F作用于乳癌细胞培养物诱导的HUVEC 24 h后,下调HUVEC KDR、Flt-1 mRNA和蛋白的表达(mRNA:F=86.381、79.175;蛋白:F=36.621、127.910,P均<0.001)。结论:5F抑制乳癌细胞培养物诱导的HUVEC体外增殖、穿膜能力、趋化性运动能力和黏附基质能力,其机制可能与下调细胞KDR mRNA和蛋白的表达水平有关。Aim：To investigate the effect and its possible mechanisms of Pteris semipinnata L extract（ 5F） on angiogenic abilities of human umbilical vein endothelial cells（ HUVEC） induced by the culture of human high metastatic breast cancer MDA-MB-231 cells. Methods：MTT assay was used to evaluate the cell proliferation of HUVEC induced by the culture of MDA-MB-231 cells after being treated by 5F for 6 h and 24 h. The effect of 5F on invasion and migration of HUVEC induced by the culture of MDA-MB-231 cells was measured by transwell chamber assay. The adhesive potential of HUVEC cells induced by the culture of MDA-MB-231 cells was tested by cell-matrix adhesion assay. RT-PCR and Western blot were applied to detect the expression levels of KDR,Flt-1 mRNA and protein in HUVEC induced by the culture of MDAMB-231 cells. Results：5F inhibited the proliferation of HUVEC induced by the culture of MDA-MB-231 cells after 6 h and 24 h treatment（ F group= 64. 852,F time= 131. 393,P 0. 001）. 20,40 and 80 μmol / L 5F significantly inhibited the invasion,migration and adhesion to matrigel of HUVEC induced by the culture of MDA-MB-231 cells（ F = 48. 206,147. 613,22. 081,P 0. 001）. After 24 h treatment,5F down-regulated the expressions of KDR,Flt-1 mRNA and protein（ mRNA：F =86. 381,79. 175; protein：F =36. 621,127. 910,P 0. 001） induced by the culture of MDA-MB-231 cells.Conclusion：5F could inhibit the proliferation,invasion,and migration of HUVEC induced by the culture of MDA-MB-231cells and adhesion to matrix of HUVEC,which may be involved in its down-regulation of the expression of KDR.

关 键 词：半边旗提取物 5F 人脐静脉内皮细胞 KDR FLT-1 血管生成 MDA-MB-231 

分 类 号：R978.7[医药卫生—药品]