艾叶乙酸乙酯提取物对HBV的抑制作用  

Anti-hepatitis B virus activities of ethyl acetate extracts from folium artemisiae argyi in vitro

作　　者：赵志鸿[1] 侯迎迎[2] 郑立运[1] 王丽阳[2] 王桂芳[1] 张小俊[1] 张壮丽[1] 

机构地区：[1]郑州大学医药科学研究院,郑州450052 [2]郑州大学药学院,郑州450001

出　　处：《郑州大学学报（医学版）》2013年第6期783-785,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省省属科研单位社会公益项目预研专项资金09yy0030

摘　　要：目的:通过体外实验观察艾叶乙酸乙酯提取物对乙型肝炎病毒(简称HBV)的抑制作用。方法:取HepG2.2.15细胞,以1.6、8.0、40.0、200.0和500.0 mg/L的艾叶乙酸乙酯提取物处理72 h后,采用MTT法测定细胞生长抑制率,计算半数毒性浓度。另取HepG2.2.15细胞,分别以0、10、20和40 mg/L艾叶乙酸乙酯提取物及100 mg/L拉米夫定(阳性对照)处理3、6、9 d,采用酶联免疫法检测细胞上清液中乙肝表面抗原、e抗原,用荧光定量PCR测定HBV DNA拷贝数,分别计算抑制率和半数抑制浓度,最后计算治疗指数。结果:艾叶乙酸乙酯提取物对HepG2.2.15细胞的半数毒性浓度为104.80 mg/L,对乙肝表面抗原、e抗原及HBV DNA的半数抑制浓度分别是1.26、8.06和38.97 mg/L,并呈剂量依赖性。治疗指数分别为HBsAg 83.2、e抗原13.0和HBV DNA 2.7。结论:艾叶乙酸乙酯提取物在体外对HBV有明显抑制作用。Aim：To observe the anti-hepatitis B virus activities of ethyl acetate extracts of folium artemisiae argyi.Methods：HepG2. 2. 15 cells were allocated into 5 groups and given ethyl acetate extracts at 1. 6,8. 0,40. 0,200. 0 and500. 0 mg / L for 72 h; the inhibition rate of cell growth was determined by MTT and TC50was calculated. Another HepG2. 2. 15 cells were allocated into 5 groups and given ethyl acetate extracts at 0,10,20 and 40 mg / L and lamivudine（ positive control） at 100 mg/L for 3,6,and 9 days; ELISA was used to determine the contents of HBsAg and HBeAg in cell supernatant,and FQ-PCR was used to determine the HBV DNA copies and the inhibition rate and IC50were calculated,and then the therapeutic index was calculated accordingly. Results：The TC50of ethyl acetate extracts was 104. 80mg / L,and the IC50for HBsAg,HBeAg and HBV DNA was 1. 26,8. 06,and 38. 97 mg / L,and the therapeutic index for the above 3 indexes was 83. 2,13. 0,and 2. 7. Conclusion：The extracts from folium artemisiae argyi have obvious inhibitory effects on hepatitis B virus in vitro.

关 键 词：艾叶 乙酸乙酯提取物 乙型肝炎病毒 HEPG2 2 15细胞 

分 类 号：R965.1[医药卫生—药理学]