沙颍河流域居民血清铅、镉、锌、铜及金属硫蛋白的测定  

Measurement and analysis of serum lead,cadmium,zinc,copper and metallothionein in residents of Shaying River area

作　　者：李仕群[1] 朱静媛[1] 李岩[1] 王志瑾[1] 袁伟[1] 程学敏[1] 巴月[1] 崔留欣[1] 

机构地区：[1]郑州大学公共卫生学院劳动卫生学与环境卫生学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2013年第6期788-791,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家"十二.五"重大水专项子课题2009ZX07210-006-3-3

摘　　要：目的:探讨沙颍河污染区外环境重金属暴露对居民血清铅、镉、锌、铜及金属硫蛋白(MT)的影响。方法:在距沙颍河河堤5 km以内和20 km以外各选取一个人口构成相似的村庄分别作为污染区和对照区,测量河水、饮用水、土壤、蔬菜和粮食中的铅、镉含量。采用火焰原子吸收光谱法测定河水、饮用水、土壤、蔬菜、粮食中的铅、镉含量;采用溶出伏安法测定各组人群血清中的铅、镉、锌、铜含量;采用ELISA方法测定人群血清中MT的含量。结果:污染区饮用水、土壤、粮食和蔬菜中的铅、镉含量均高于对照区(铅:t=2.663、2.300、3.001和2.117,P<0.05;镉:t=3.549、2.073、2.202和2.167,P<0.05);污染区人群的血清铅、镉水平和铜/锌比值高于对照区(t=5.544、10.438和5.556,P<0.05);血清MT含量随着血清铅、镉含量的增加而增加。结论:沙颍河水污染已影响到当地居民体内的重金属负荷。Aim：To investigate the influence of heavy metal exposure of Shaying River area on the serum lead,cadmium,zinc,copper and metallothionein（ MT） levels in residents of the area. Methods：Contaminated and control areas were chosen from villages less than 5 km and more than 20 km away from the Shaying River respectively,which had similar population composition and economy condition. The concentrations of lead and cadmium in drinking water,soil,grain and vegetables samples from the two areas were measured respectively by flame atomic absorption spectrometry. Serum lead,cadmium,zinc and copper levels were determined by cyclic voltammetry method. Serum MT level was measured by ELISA.Results：The levels of serum lead,cadmium in drinking water,soil,grain and vegetable samples in the contaminated area were significantly higher than those of control area（ lead：t = 2. 663,2. 300,3. 001,and 2. 117,P 0. 05; cadmium：t =3. 549,2. 073,2. 202,and 2. 167,P 0. 05）. The levels of serum lead,cadmium,zinc,and Cu / Zn in the contaminated area were significantly higher than those of control area respectively（ t = 5. 544,10. 438,and 5. 556,P 0. 05）. Conclusion：Water pollution of Shaying River has affected the load of heavy metals in local residents.

关 键 词：水污染 血铅 血镉 金属硫蛋白 

分 类 号：R123.1[医药卫生—环境卫生学]