益肺活血复方对人肺动脉内皮细胞微结构及内分泌功能的影响  

Effects of Compound Yifei Huoxue on cell microstructure and secretion function of human pulmonary artery endothelial cells

作　　者：王婷[1] 欧敏[1] 黄友章[1] 

机构地区：[1]中国人民解放军海军总医院干部呼吸科,北京100048

出　　处：《郑州大学学报（医学版）》2013年第6期799-802,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：全军医药卫生科研基金资助项目36040

摘　　要：目的:观察益肺活血复方对人肺动脉内皮细胞微结构及内分泌功能的影响。方法:建立人肺动脉内皮细胞低氧高二氧化碳模型;制备益肺活血复方不同给药剂量(生药10、20、40 g/kg)的含药血清;在细胞培养液中共同孵育12 h后,利用透射电镜观察细胞微结构变化,采用放射免疫法测定各组细胞培养液上清中内皮素-1(ET-1)及前列环素(PGI2)的含量,采用硝酸还原酶法测定细胞培养液中一氧化氮(NO)的含量。结果:低氧高二氧化碳条件下,人肺动脉内皮细胞微结构损伤,而中、高剂量益肺活血复方组细胞微结构损伤减轻。各组细胞培养液或培养液上清中ET-1、NO、PGI2的含量比较,差异有统计学意义(F=121.870、60.040、49.060,P均<0.001);模型组ET-1含量高于空白对照组,NO、PGI2含量低于空白对照组(P均<0.01);中、高剂量益肺活血复方组ET-1含量低于模型组,NO和PGI2含量高于模型组(P均<0.01)。结论:低氧高二氧化碳导致人肺动脉内皮细胞微结构及功能障碍,而中、高剂量益肺活血复方可减轻其结构及功能损伤的程度。Aim：To investigate the effects of Compound Yifei Huoxue（ CYH） on the cell microstructure and secretion function of human pulmonary artery endothelial cells. Methods：A model of hypoxia hypercapnia was established. Different doses of pharmacy（ 10,20,40 g / kg crude drug） were added into the culture medium to prepare serum containing CYH.After incubation for 12 h,the cell microstructure was observed by transmission electron microscopy,the levels of ET-1 and PGI2were tested by radioimmunoassay method,and NO level was tested by nitrate reductase. Results：Hypoxic hypercapnia caused damage to the cell microstructure,while moderate and high dose CYH could alleviate the damage. There were significant differences in the levels of ET-1,NO and PGI2among 3 CYH model group and blank control group（ F =121. 870,60. 040,49. 060,P 0. 001）. The level of ET-1 in the model group was higher than that of blank control group,but the levels of NO and PGI2were lower（ P 0. 01）. The levels of ET-1 in moderate and high dose CYH groups were lower than that of model group,but the levels of NO and PGI2were higher（ P 0. 01）. Conclusion：Hypoxia hypercapnia could cause human pulmonary artery endothelial cell microstructure damage and dysfunction. Moderate and high dose CYH could alleviate the damage.

关 键 词：益肺活血复方 低氧高二氧化碳 人肺动脉内皮细胞 细胞微结构 内皮素-1 一氧化氮 前列环素 

分 类 号：R965[医药卫生—药理学]