2010-2011年苏州地区腹泻婴幼儿感染人博卡病毒的分子特征研究  

Molecular features of human bocavirus from infantile diarrhea in Suzhou area, Jiangsu province in 2010-2011

作　　者：付建光[1] 蒋翠莲[2] 秦圆方[1] 艾静[1] 刘成[3] 吴斌[1] 祁贤[1] 鲍昌俊[1] 朱叶飞[1] 汤备扬 

机构地区：[1]江苏省疾病预防控制中心卫生部肠道病原微生物重点实验室,南京210009 [2]南京市妇幼保健院 [3]苏州市疾病预防控制中心

出　　处：《中华流行病学杂志》2013年第12期1216-1218,共3页Chinese Journal of Epidemiology

基　　金：江苏省“十二五”科教兴卫工程（ZX201109）;江苏省医学重点人才基金（RC2011084,RC2011085）

摘　　要：目的明确苏州地区腹泻婴幼儿博卡病毒感染情况、病毒主要基因型剔及其流行病学特征。方法收集2010—2011年苏州市儿童医院疑似病毒性腹泻患儿粪便标本832例（份），采用荧光定量PCR方法检测粪便巾博卡病毒DNA，并对部分阳性标本测序明确病毒基因型。结果832份粪便标本检出博卡病毒阳性51份，阳性率为6．1％。7—9月为博卡病毒感染高峰；96％的患儿为〈2岁，其中7～12月龄为高发年龄段；男性患儿多于女性；36例合并其他腹泻病毒感染。对35份博卡病毒阳性标本进行测序，其中28份为HBoV-1型，5份为HBoV-2型，2份型刖未确定，未发现HBoV-3型及HBoV-4型。结论苏州地区婴幼儿腹泻标本中博卡病毒以HBoV-1型为主，同时存在HBoV-2型感染。Objective To investigate the status of human bocavirus and to indentify its epidemiological characteristics as well as genotype distribution in patients with infantile viral diarrhea in Suzhou, Jiangsu province. Methods 832 fecal specimens from patients with infantile virus diarrhea cases were collected from Suzhou Children＇ s Hospital in 2010-201 l. Human bocavirus were detected by Real-Time RT-PCR, and genotype were determined by sequence analysis. Results Among all the fecal specimens, 51 （6.1% ） cases were positive for human bocavirus. The peak season of rotavirus infection was between July and September. Of all the episodes on rotavirus diarrhea, 96% occurred before 2 years of age, with peaks in children with 7-12 months of age. Data from Nucleotide Sequence analysis showed that among 28 samples that carrying HBoV-1, 5 strains belonged to HBoV-2, HBoV type 3 but type 4 were absent. Conclusion Human bocavirus were detected from fecal specimens of infantile virus diarrhea in Suzhou, with genotype HBoV-1 as the major strain. HBoV-2 genotype was also found.

关 键 词：博卡病毒 基因型 婴幼儿腹泻 

分 类 号：R723.11[医药卫生—儿科] R181.3[医药卫生—临床医学]