基于哮喘小鼠模型研究生、制百部醇提物作用差异  

Difference in effect between asthma-based mouse model and Stemona tuberosa extracts

作　　者：陈晓霞[1,2] 张晓丹[1] 李红艳[1] 贾天柱[1,3] 杨静娴[1] 

机构地区：[1]辽宁中医药大学,辽宁大连116600 [2]辽宁省中药炮制工程技术研究中心,辽宁大连116600 [3]国家中医药管理局中药炮制重点研究室,辽宁大连116600

出　　处：《中国中药杂志》2013年第23期4084-4087,共4页China Journal of Chinese Materia Medica

基　　金：2010;2011年中医药行业科研专项(201107007)

摘　　要：该文以OVA激发小鼠哮喘为模型,分别口服灌胃给予不同浓度生、制百部醇提取物,测定其脾细胞中Th1及Th2释放的细胞因子,采用RT-PCR技术测定实验小鼠肺组织中GATA-3及T-bet基因表达,比较生、制百部醇提取物治疗小鼠哮喘作用的差异性。结果发现与生品比较,制品给药后更能上调哮喘小鼠脾细胞中炎症因子IFN-γ(P<0.05)及下调IL-5(P<0.05);RT-PCR结果发现制品给药后更能上调T-bet/GATA-3(P<0.05)表达。该实验表明生、制百部醇提取物均通过调节Th1/Th2比例治疗哮喘,但制品作用更为显著,说明百部炮制前后其药效学上存在显著差异,因此临床治疗哮喘及慢性咳嗽时以制品入药更合理,为进一步揭示百部炮制原理奠定基础。In this study, OVA-induced asthma mice was taken as the model, and orally administered with different concentra- tion of ethanol extracts of crude and processed Stemona tuberosa, in order to determine the cytokine level released from Thl and Th2 in splenocytes. RT-PCR was carried out to determine the genetic expression of T-bet/GATA-3 in lung, and compare the differentiation be- tween ethanol extracts of crude and processed S. tuberosa in therapeutic effect on asthma in mice. According to the results, compared with the crude samples, processed samples significantly increased the levels of inflammatory factor INF-T （P 〈 0. 05） and decreased IL- 5 （P 〈 0. 05） in splenocytes. According to the RT-PCR results, the administration of processed samples could increase the ratio of T-bet/GATA-3 （P 〈 0. 05 ）. The experiment showed that ethanol extracts of both crude and processed S. tuberosa could treat asthma by regulating Thl/Th2 ratio, but processed samples showed more notable effect. This indicated that crude and processed S. tuberosa had significant pharmacological difference. Therefore, it was more rational to apply processed S. tuberosa in clinical treatment of asthma and chronic cough, which layed a foundation for further revealing the processing mechanism of S. tuberosa.

关 键 词：生 制百部 哮喘 细胞因子 GATA-3 T—Bet 

分 类 号：R285.5[医药卫生—中药学]