淫羊藿女贞子配伍对维甲酸致大鼠骨质疏松模型的影响研究  

Study on effect of combination of Epimedii Folium and Ligustri Lucidi Fructus on osteoporosis rats induced by retinoic acid

作　　者：康学[1] 李崭[1] 张伟华[2] 周琦[3] 刘仁慧[1] 王秀娟[1] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]上海中医药大学附属岳阳中西医结合医院,上海200437 [3]首都医科大学附属北京中医医院,北京100010

出　　处：《中国中药杂志》2013年第23期4124-4128,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81373814);北京市教育委员会科技计划面上项目(KM201310025012);首都中医药及护理学研究专项课题(12ZYH07);北京中医药薪火传承"3+3"工程二室一站建设项目(2012-SZ-C-42)

摘　　要：目的:探讨淫羊藿女贞子配伍对维甲酸致大鼠骨质疏松模型的影响。方法:3月龄雄性Wistar大鼠60只,随机分为正常对照组、模型对照组、淫羊藿组、女贞子组、淫羊藿女贞子配伍组、雷洛昔芬组。维甲酸灌胃2周建立大鼠骨质疏松模型,造模同时各给药组分别灌服相应药物治疗3周。测定血清钙(Ca)、磷(P)、碱性磷酸酶(AKP)、抗酒石酸酸性磷酸酶(StrACP)含量,并观察股骨病理形态学变化。结果:模型组较对照组血清Ca,P含量显著降低,AKP,StrACP活性均显著升高,股骨头区骨小梁数目减少,窄小稀疏,断裂,呈典型的骨质疏松样改变;与模型组比较,各给药组均可显著提高血清Ca,P含量,下调血清AKP,StrACP水平,且骨小梁数目增加,变宽,密集交织呈网状,以配伍组作用最为显著。结论:淫羊藿女贞子可有效纠正骨代谢异常,改善骨组织病理状态,从而发挥抗骨质疏松作用,其中以二药配伍应用效果更佳。Objective： To explore the effect of combination of Epimedii Folium and Ligustri Lucidi Fructus on osteoporosis rats induced by retinoic acid. Method： Sixty three-month-old male Wistar rats were randomly divided into the noruml control group, the model group, the Epimedii Folium group, the Ligustri Lucidi Fructus group, the combination group of Epimedii Folium and Ligustri Lucidi Fructus and the raloxifene group. The osteoporosis model was established through oral administration with retinoic acid for two weeks. Meanwhile, all of treatment groups were administered with corresponding drugs for three weeks. The contents of serum calcium （ Ca）, phosphorus （ P）, alkaline phosphatase （AKP） and tartrate-resistant acid phosphatase （StrACP） were detected, and the patho- morphological changes of femurs were observed. Result： The model control group showed much lower contents of serum Ca and P than the normal control group, but with significantly higher AKP and StrACP activity than the normal control group. The femoral head area showed reduced, narrow and sparse trabecular bones, with typical osteoporosis-like changes. Compared with the model control group, all of treated groups showed significant increase in Ca and P contents in serum, and down-regulate AKP and StrACP levels, while tra- becular bones became more and wider, and densely inte,＇weaved as a reticular formation. Among them, the combination group showed the most significant effect. Conclusion： Epimedii Folium and Ligustri Lucidi Fructus could effectively correct the abnormal bone me- tabolism and improve pathological conditions of bone tissues, so as to show the anti-osteoporosis effect. The combined application of the two drugs showed a better efficacy.

关 键 词：骨质疏松 维甲酸 淫羊藿 女贞子 实验研究 

分 类 号：R285.5[医药卫生—中药学]