小型猪痰瘀互结证冠心病“痰、毒、瘀”病机演变规律的实验研究  

Experimental study on pathogenetic evolvement regularity of phlegm,toxin and blood-stasis syndromes in Chinese miniswine with phlegm-stasis cementation syndrome of coronary heart disease

作　　者：刘建勋[1] 林成仁[1] 任建勋[1] 李磊[1] 任钧国[1] 付建华[1] 刘光宇[1] 

机构地区：[1]中国中医科学院西苑医院基础医学研究所、中药药理北京市重点实验室,北京100091

出　　处：《中国中药杂志》2013年第23期4138-4143,共6页China Journal of Chinese Materia Medica

基　　金：国家“重大新药创制”科技重大专项(2009ZX09502-017,2012ZX09301002-004);北京市科技专项;中药药理北京市重点实验室2012年阶梯计划项目(z121107002812036)

摘　　要：目的:探讨小型猪痰瘀互结证冠心病病机演变的规律。方法:将18只中国小型猪随机分为正常对照组,模型组,丹蒌片组,每组6只。除正常对照组外,其他各组高脂饲料喂养2周后,采用介入技术球囊损伤冠状动脉左前降支内皮,继续高脂饲料喂养8周,制备小型猪痰瘀互结证冠心病模型。于0周(实验前)、高脂2周(术前或给药前)、高脂6周(给药后4周)、高脂10周(给药后8周),分别观察各组动物体重指数(BMI)、血液流变性参数、血脂水平、血清炎症因子水平的动态变化,并在实验结束时观察肝脏甘油三酯(TG)和总胆固醇(TC)水平及冠状动脉组织病理改变。结果:与对照组比较,模型组自第2周起至实验结束,血清TC,TG,LDL-C及VLDL-C水平均升高(P<0.01),5 s-1和60 s-1切变率下的全血黏度均明显增加(P<0.05或P<0.01);第6周时BMI和血清TNF-α水平均明显升高;第10周时BMI及血清hs-CRP,IL-6,TNF-α水平均明显升高,同时冠脉管腔狭窄率、内膜厚度,及内膜厚度/中膜厚度比值均明显增加(P<0.05或P<0.01);肝脏TC和TG水平明显升高(P<0.01)。与模型组比较,丹蒌片组的冠状动脉病变程度明显减轻,其内膜厚度、管腔狭窄率,及内膜厚度/中膜厚度比值均明显减少(P<0.01),BMI,血清TC,TG,LDL-C,VLDL-C,肝脏TC,TG,以及血清hs-CRP,IL-6,TNF-α水平亦均明显降低(P<0.05或P<0.01),5 s-1和60 s-1切变率下的全血黏度均明显降低(P<0.05)。结论:痰浊、邪毒、瘀血三者的相互影响促进动脉粥样硬化(AS)斑块的发生和发展,是痰瘀互结证冠心病的关键病机所在。Objective： To discuss that pathogenesis evolvement regularity of Chinese miniature swine with phlege-stasis cemen- tation syndrome of coronary heart disease. Method： Eighteen Chinese miniature swine were randomly divided to the normal control group, the model group and the Danlou tablet group, with six swine in each group. Except for the normal control group, all of the other groups were fed with high fat diet for two weeks. The coronary heart disease model with phlegm-stasis cementation syndrome was estab- lished by injuring left anterior descending artery with interventional balloons and continuously feeding with high fat diet for eight weeks. The levels of BMI, hemorheological parameters, lipids in serum and inflammatory cytokines were observed at the 0th （ before the exper- iment）, 2nd （before operation or drug administration）, 6th （four weeks after drug administration） and 10th week （eight weeks after drug administration） of study. The levels of TG and TC in liver and the pathological changes in coronary artery tissues were also ob- served at the end of study. Result： Compared with the normal control group, the model group had showed significant increase in the levels of TC, TG, LDL-C and VLDL-C in serum （P 〈0. 01 ） from the second week to the end of the experiment, with notable rise in the whole blood viscosity under the shear rates of 5 s -1 and 60 s-1. At the 6th week, the levels of BMI and TG and TNF-a in serum significantly increased. At the 10th week, the levels of BMI and hs-CRP, IL-6 and TNF-α in serum significantly increased as well, with remarkable increase in coronary stenosis, intimal thickness and the ratio between intimal thickness and media thickness （P 〈 0. 05 and P 〈 0. 01 ） , and significant rise in TC and TG in livers （ P 〈 0. 01 ）. Compared with the model group, the Danlou tablet group showed obvious reduction in severity of coronary artery lesion, iutimal thickness and lumen stenosis ratio and ratio between intimal thickness and media thickness

关 键 词：冠心病 中国小型猪 痰瘀互结 毒邪 病机规律 

分 类 号：R259[医药卫生—中西医结合]