寒凉药对热证大鼠肝线粒体蛋白质组的影响  

Effect of cold and cool herbs on liver mitochondria proteome of rats with heat symptom

作　　者：李毅[1] 卢德赵[1] 唐利华[1] 沃兴德[1] 杨贞[1] 

机构地区：[1]浙江中医药大学生命科学学院,浙江杭州310053

出　　处：《中国中药杂志》2013年第23期4144-4147,共4页China Journal of Chinese Materia Medica

基　　金：浙江省中医药管理局重点项目(2006Z002);浙江省中医药管理局项目(2007CB139)

摘　　要：自20世纪60年代起,现代科学开始对热证的本质进行研究,但有关热证的病理机制和寒凉药物的作用机制等尚未深入系统研究。该研究以具有代表性的热性中药喂饲动物造成热证模型,后用寒凉药进行实验性治疗,拟采用双向电泳及质谱分析技术对热证模型大鼠及寒凉药治疗后的大鼠肝线粒体蛋白组进行比较,从中发现热证及寒凉药治疗后的特异性相关蛋白质。In the 1960s, modem science began involving the essence of heat syndrome, but there have still no in-depth sys- tematic studies on pathological mechanisms of heat syndrome and action mechanisms of cold and cool herbs. In this study, the animal model with heat syndrome was set up by feeding herbs with hot property, and then cold and cool herbs was applied in the experimental therapy. The two-dimensional electrophoresis and mass spectrometry technologies were adopted to compare the liver mitochondria pro- teome of the rats of the heat syndrome model and the ones treated with cold and cool herbs, so as to discover specificity-related proteins after heat syndrome and treatment with cold and cool herbs.

关 键 词：寒凉药 热证 线粒体 蛋白质组 

分 类 号：R285.5[医药卫生—中药学]