大豆子叶RNA和DNA的同步抽提及质量分析  

Simultaneous Isolation of RNA and DNA from Soybean Cotyledon and Analysis of Their Quality

作　　者：周向红[1] 王萍[1] 

机构地区：[1]淮海工学院海洋学院,江苏连云港222005

出　　处：《作物杂志》2013年第6期36-39,共4页Crops

基　　金：中央财政支持地方高校发展专项资金资助(CXTD07);教育部大豆重点实验室开放课题(SB08A03)

摘　　要：本文旨在建立一种适用于大豆子叶的RNA和DNA同步抽提方法,得到高质量的核酸以满足后续实验要求。该方法先用异硫氰酸胍裂解大豆子叶,再用常规苯酚/氯仿抽提去除杂质,最后用LiCl选择性沉淀核酸。核酸的完整性和纯度检测表明抽提的总RNA和DNA质量良好,进一步的RT-PCR和PCR检测显示抽提的总RNA和DNA质量适合后续的实验要求。A method for simultaneous isolation of RNA and DNA is desirable when a limited amount of sample is available. The purpose of this paper was to present a new method to isolate DNA and RNA simultaneously from soybean cotyledon （Glycine max）. Sample was first lysed and homogenized in liquid nitrogen and the presence of a guanidine isothiocyanate-containing buffer. The lysed mixture was then extracted by a phenol/chloroform mixture e- quilibrated with Tfis-HC1 buffer. The nucleic acids （DNA and RNA） were differentially precipitated by the addition of LiC1 and ethanol. The isolated nucleic acids had a good integrity and purity and were suited for the further experiments.

关 键 词：大豆子叶 同步抽提 RNA DNA 

分 类 号：S565.1[农业科学—作物学]