河南省甘薯茎腐病的分离与鉴定  

Molecular Identification of a New Bacterial Stem Rot Disease of Sweetpotato in Henan

作　　者：秦素研 黄立飞[2] 葛昌斌 刘志坚 张勇跃 李建国 房伯平[2] 

机构地区：[1]漯河市农业科学院,河南漯河462300 [2]广东省农业科学院作物研究所,广东广州510640

出　　处：《作物杂志》2013年第6期52-55,I0002,共5页Crops

基　　金：国家甘薯产业技术体系项目(CARS-11-C-13)

摘　　要：2012年在河南省襄城县甘薯产区发现了一种引起甘薯快速死苗的甘薯病害,主要症状表现为叶片变黄,茎基部呈黑色水浸状腐烂,并逐渐沿茎枝向顶端腐烂,后整株倒伏、死亡。从病地采集受害甘薯茎蔓后分离纯化获得了病原菌,经柯赫氏法则验证及16S rDNA序列分析,鉴定其为菊欧文氏菌(Erwinia chrysanthemi)甘薯茎腐病。这是河南省首次发现该病害。A suspected new disease of sweetpotato was observed at a number of sweetpotato areas in Henan Province in 2012. The disease symptoms often appear as yellow leaves and black water soaked rot extending from the bottom to the top of stems resulting in collapse and death of the entire plant. Bacterial strains were isolated from the diseased seedling stems of sweetpotato. In this study, the bacteria isolated from sweetpotato disease stems was identi- fied as Erwinia chrysanthemi by Kochs postulate validation and 16S rDNA sequence analysis. This is the first report of bacterial stem and root rot of sweetpoato in Henan Province.

关 键 词：甘薯 茎腐病 菊欧文氏菌 分子鉴定 

分 类 号：S435.31[农业科学—农业昆虫与害虫防治]