外源水杨酸对铝胁迫下吊瓜抗氧化系统的影响  

Effects of exogenous salicylic acid on Trichosathes kirilowii Maxim antioxidant system under aluminum stress

作　　者：章艺[1] 周媛[2,3] 吴玉环[4] 郑人卫[5] 罗虹[2] 徐根娣[2,3] 高培培[3] 姚国浩[3] 刘鹏[2,3] 

机构地区：[1]浙江旅游职业学院,杭州311231 [2]浙江师范大学生态学研究所,金华321004 [3]浙江师范大学植物学实验室,金华321004 [4]杭州师范大学生命与环境科学学院,杭州310036 [5]浙江师范大学物理化学研究所,金华321004

出　　处：《安徽农业大学学报》2013年第6期1039-1042,共4页Journal of Anhui Agricultural University

基　　金：浙江省自然科学基金(Y5100390);浙江省公益技术研究农业项目(2011C22053)共同资助

摘　　要：吊瓜,学名栝楼(Trichosathes kirilowii Maxim),是具有药食两用特性的经济作物。为了研究水杨酸(SA)对吊瓜铝(Al)胁迫的缓解效应,以长兴仁吊瓜为试验材料,用溶液培养法,在500μmol L-1Al胁迫下,喷施不同浓度SA,并对其生长特性、过氧化氢(H2O2)、丙二醛(MDA)含量及抗氧化酶活性等指标进行测定。结果显示,Al胁迫下,吊瓜根长、根鲜重及地上部分鲜重明显降低,植株超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、愈创木酚过氧化物酶(G-POD)等抗氧化酶活性降低,抗坏血酸过氧化物酶(APX)活性升高,过氧化氢(H2O2)含量及丙二醛(MDA)含量提高;喷施一定浓度SA后,根系生长抑制解除,SOD、CAT及G-POD抗氧化酶类活性升高,MDA和H2O2含量降低。SA浓度为10μmol·L-1时,效果更佳。由此可知,外源SA处理能够诱导Al胁迫下吊瓜的抗氧化反应,SA是一种能够缓解吊瓜Al胁迫的有效物质,具有推广应用价值。Salicylic acid （SA）, a plant phenolic, is now considered as a hormone-like endogenous regulator, and its role in the defense mechanisms against biotic and abiotic stress has been documented. The aim of this study was to investigate the impact of exogenous application of SA （10μmol·L^-1, 50μmol·L^-1） on the growth characteristics, activities of anti-oxidant enzymes, H202 and MDA contents of Trichosathes kirilowii Maxim ex- posed to A1CI3 （500 μmol·L^-1）. The seedlings were treated for 20 days, and then the parameters including the growth and the activities of antioxidant enzymes, the contents of hydrogen peroxide and malonaldehyde in root were measured. The results were as follow： with the treatment of A1, the activities of SOD, CAT and G-POD decreased significantly, but the activities of APX increased remarkably, and the contents of H2O2 and MDA increased significantly. We found that exogenous SA significantly reduced the contents of H2O2 and MDA, while increased the activities of SOD, CAT and G-POD significantly. The activity of APX closed to the normal level by sprinkling exogenous SA. Besides, 10 μmol·L^-1 SA could promote the growth of roots, which were expressed in root length, and increased the fresh weight. These results indicated that exogenous SA partially involved in part, in inducing anti-oxidative defense system in aluminum stress Trichosathes kirilowii Maxim. In general, of the two concentrations tested, 10μmol·L^-1 SA had greater effect throughout the experiment. The results showed that the external source of SA could alleviate aluminum toxicity to some extent. It is predicted that SA might be used as a pragmatic approach to improve aluminum tolerance in crops.

关 键 词：吊瓜 铝胁迫 水杨酸 抗氧化系统 

分 类 号：S154.4[农业科学—土壤学] Q949.91[农业科学—农业基础科学]