甲异靛诱导K562细胞凋亡及对传导信号STAT5表达影响的研究  

The research on meisoindigo accelerating apoptosis of K562 cells And its effect on the expression of conduction signal STAT5

作　　者：周迎春[1] 刘基铎[1] 谢在春[1] 徐倩[1] 肖明锋[1] 

机构地区：[1]广州中医药大学第一附属医院检验科,510405

出　　处：《国际中医中药杂志》2013年第12期1076-1078,共3页International Journal of Traditional Chinese Medicine

基　　金：广东省科技厅资助项目（项目编号：2011B031700040）

摘　　要：目的 探讨甲异靛诱导K562原代细胞凋亡及对传导信号分子STAT5表达影响的作用.方法 K562细胞甲异靛处理12h、24 h、48 h后,采用流式细胞术检测细胞凋亡及细胞周期等相关指标,Western blot检测STAT5的改变.结果 ①甲异靛作用K562细胞12h、24 h、48 h凋亡率分别为12.5±0.69,19.4±1.07和42.5±3.22,与对照组相同时间段比较,差异均有统计学意义（P＜0.05）.②甲异靛可使K562细胞阻滞于S期,S期细胞比例增加至（70.48±6.34）,G0/G1期、G2/M期细胞减少.③在甲异靛作用12 h后至48 h,STAT5蛋白含量逐渐减少.结论 甲异靛促进K562细胞凋亡,可能与STAT5蛋白含量表达减少有关.Objective To investigate the effect of meisoindigo inducing primary K562 cell apoptosis and its influence on the expression of conduction signal STAT5.Methods K562 cell was treated with meisoindigo for 12 h、24 h、48 h respectively.Flow cytometry was used to detect cell apoptosis and cell cycle,and Western blot was employed to examine the alteration of the expression of STAT5.Results ①The rate of K562 cell apoptosis treated with meisoindigo for 12 h、24 h、and 48 h was 12.5 ± 0.69,19.4± 1.07 and 42.5 ± 3.22,showing statistical significance compared with the control group （P〈0.05）.②Meisoindigo could block K562 cell at the S stage,the ratio of S stage cell was increased to 70.48±6.34 with the decrease of cells at G0/G1 and G2/M stage.③Gradually reduced expression of STAT5 showed after K562 cells treated with meisoindigo from 12h to 48h.Conclusion Meisoindigo accelerrates the apoptosis of K562 cell and the mechanism may relevant to the decreased expression of STAT5.

关 键 词：甲异靛 慢性粒细胞白血病 细胞凋亡 

分 类 号：R285[医药卫生—中药学]