痰瘀同治方对心肌缺血再灌注损伤大鼠心肌Rho/Rho激酶通路的影响  

Effect of Tanyu-Tongzhi recipe on Rho/Rho kinase signal transduction pathway of myocardial ischemia-reperfusion injury in rats

作　　者：唐丹丽[1] 佟琳[2] 崔海峰[1] 隋宇[1] 张华敏[2] 

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]中国中医科学院中医药信息研究所

出　　处：《国际中医中药杂志》2013年第12期1082-1085,共4页International Journal of Traditional Chinese Medicine

基　　金：北京市自然科学基金（项目编号：7122121）;中国中医科学院基本科研业务费自主选题项目（项目编号：zz2011007）

摘　　要：目的 观察痰瘀同治方对高脂大鼠心肌缺血再灌注损伤（myocardial ischemic-reperfusion injury,MI/RI）Rho/Rho激酶信号转导通路的影响.方法 采用随机数字表法将60只SD雄性大鼠随机分为假手术组、模型组、西药对照组及痰瘀同治方高、低剂量组.可逆性冠脉左前降支结扎缺血30 min 再灌注2h复制MI/RI模型,应用RT-PCR及Western blotting法检测各组大鼠心肌RhoA、ROCK Ⅱ mRNA 表达及ROCK下游底物肌球蛋白轻链（MLC）磷酸化水平.结果 与模型组比较,痰瘀同治方低剂量组可降低心肌RhoA、ROCKⅡmRNA及磷酸化MLC （p-MLC）水平（P值分别为0.045、0.042、0.023,P均＜ 0.05）.结论 痰瘀同治方可通过抑制RhoA、ROCKⅡ激活及p-MLC蛋白过度表达而调控Rho/Rho激酶信号转导通路,发挥其对缺血再灌注损伤心肌的保护作用.Objective To investigate the influence of Tanyu-Tongzhi （TYTZ）recipe on Rho/Rho kinase signal transduction pathway of myocardial ischemia-reperfusion injury in high fat-fed rats.Methods 60 SD rats were divided into 5 groups randomly,a sham-operated group,a model group,a western medicine control group,a high-dose group and a low-dose group of TYTZ recipe.The model of ischemia reperfusion of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats.The downstream substrates of ROCK myosin light chain （MLC） phosphorylation expression of myocardial tissues in rats were detected by Western blotting method.The levels of RhoA,ROCK Ⅱ mRNA were determined with RT-PCR.Results Compared with the model group,the expression of p-MLC and RhoA,ROCK Ⅱ mRNA were lower in low-dose group of TYTZ,there were significant differences between the two groups（P=0.004、0.003、0.018、0.004,P〈0.01）.Conclusion The TYTZ Recipe can protect myocardium from MI/RI.The mechanism of action was related to its inhibiting the protein expression of RhoA,ROCK Ⅱ and p-MLC,restraining the activation of Rho/Rho kinase signal transduction pathway.

关 键 词：痰瘀同治方 心肌缺血再灌注损伤 信号转导通路 

分 类 号：R285.5[医药卫生—中药学]