采用诱导分化的SH-SY5Y细胞建立酒精性痴呆体外研究模型的探讨  

Setting up alcohol-associated dementia in vitro model with RA-induced differentiated SH-SY5Y cell

作　　者：杨海玉[1] 刘勇[2] 

机构地区：[1]江西省人民医院临床医学研究所江西省神经病学研究所,江西南昌330006 [2]江西省人民医院病理科,江西南昌330006

出　　处：《中风与神经疾病杂志》2013年第11期971-973,共3页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金项目(No.81060111)

摘　　要：目的建立酒精诱导神经元凋亡的体外模型,探讨酒精性痴呆的发病机制。方法以SH-SY5Y细胞为实验对象,通过维甲酸(RA)诱导细胞分化,之后以不同浓度的酒精作用于细胞,连续培养24h。四甲基偶氮唑蓝(MTT)比色法检测细胞增殖;碘化丙啶(PI)染色观察细胞凋亡。结果 RA诱导分化后SH-SY5Y细胞表现神经元形态特征,酒精抑制SH-SY5Y细胞增殖并呈浓度依赖关系(P<0.001)。酒精(100mol/L)作用24h后明显诱导细胞凋亡。结论分化后SH-SY5Y细胞对酒精诱导损伤敏感,可用于建立酒精性痴呆体外研究模型。Objective To study the effect of ethanol on induction of apoptosis in differentiated SH-S^5Y cells. Methods SH-SY5Y cells were induced to differentiation with administration of retinoie acid （RA） for 6 days. Then cells were tt：eated with different doses of ethanol for 24h. The growth and proliferation of SH-SY5Y cells were analyzed with MT]＂ assay. The staining with pidium iodide was used to observe the cell apoptosis. Results The cell proliferation was inhibited by ethanol in the dose-dependent manner. Inceased apoptosis cells were detected when cells were treated with 100mol/L concentration of ethanol. Conclusions Ethanol administration may contribute to the induction of apoptosis of SH-SY5Y cells. The model that ethanol induced-SH-SY5Y cell apoptosis may be used for the research of the mechanism of alcohol-as- sociated dementia.

关 键 词：SH—SY5Y细胞 分化 酒精性痴呆 凋亡 体外研究 

分 类 号：R595.6[医药卫生—内科学]