β-环糊精-4-苄氧基苯酚包合物与DNA的相互作用  

Interactions between β-Cyclodextrin-4-Benzyloxyphenol Inclusion Compound and Herring Sperm DNA

作　　者：龙俊[1] 段雷雨 王兴明[1] 杨欢[1] 

机构地区：[1]西南科技大学材料科学与工程学院化学系,四川绵阳621010

出　　处：《中山大学学报（自然科学版）》2013年第6期86-92,共7页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目(30973634)

摘　　要：以溴化乙锭(EB)为分子探针,生理pH=7.4环境下,分别采用荧光光谱法、化学热力学法和黏度法等手段研究了β-环糊精-4-苄氧基苯酚(β-CD-PBP)包合物与鲱鱼精DNA(hsDNA)的相互作用。利用摩尔比法测定β-环糊精(β-CD)与4-苄氧基苯酚(PBP)的包合比nβ-CD∶n PBP=1∶1,其包合常数K f=7.39×103L·mol-1。β-CD-PBP包合物与hsDNA的结合比n DNA∶nβ-CD-PBP=1∶9,结合常数为K300.15K=2.86×103L/mol,K310.15K=2.69×104L/mol。热力学函数Δr H mθ=1.44×105J/mol,Δr G mθ300.15K=-1.97×104J/mol,Δr S mθ=549.32 J/(mol·K),结果表明β-CD-PBP包合物与DNA作用是熵驱动,β-CD-PBP与hsDNA的作用方式为静电和部分嵌插的作用方式。Ethidium bromide （EB） was used as molecule probe,the interaction between β-cyclodex-trin-4-Benzyloxypheno （β-CD-PBP）and herring-sperm DNA was studied in physiological buffer solution （pH=7.4）by fluorescence spectroscopy,thermodynamic analysis and viscosity method.Based on the mole ratio method and linear method,the parameters were determined as follows：the binding ratio nβ-CD∶nPBP=1∶1 and binding constant Kf=7.39 ×1 03 L·mol-1 .The binding ratio was nDNA∶nβ-CD-PBP=1∶9,the thermodynamic perameters were ΔrHmθ=1.44 ×1 05 J/mol,ΔrGmθ300.15K=-1.97 ×1 04 J/mol,ΔrSmθ=549.32 J/mol·K. ΔrSmθwas the primary driven power of this interaction,the binding constants were K300.15K=2.86 ×1 03L/mol,K310.15K=2.69 ×1 04L/mol.These results indicated that the interaction between β-CD-PBP and herring-sperm DNA belonged to electrostatic and part intercalation.

关 键 词：4-苄氧基苯酚 作用方式 β--环糊精 鲱鱼精DNA 

分 类 号：O629.74[理学—有机化学]