罗氟司特对百草枯诱导的小鼠肺、肝、肾组织损伤与纤维化的抑制作用  

Roflumilast Attenuates Paraquat-Induced Injury and Fibrosis in Lung,Liver and Kidney Tissues of Mice

作　　者：陈妍[1] 贺利利[1] 罗钰龙 林锋[1] 黄志强[1] 彭维[1] 苏薇薇[1] 李沛波[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275

出　　处：《中山大学学报（自然科学版）》2013年第6期124-128,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省教育部科技部产学研结合项目资助项目(2011A091000008)

摘　　要：为研究罗氟司特对百草枯(paraquat,PQ)中毒诱导的小鼠肺、肝、肾等器官组织损伤与纤维化的抑制作用,用昆明种小鼠腹腔注射PQ 20 mg/kg造模,给药组每天灌胃5 mg/kg地塞米松或罗氟司特,共给药21 d,造模结束后手术分离小鼠肺、肝、肾等器官,进行HE染色和Masson染色分析,观察肺、肝、肾等器官组织损伤和纤维化变化。结果发现腹腔注射PQ 20 mg/kg显著诱导了小鼠肺、肝、肾等器官的组织损伤与纤维化病变。罗氟司特组小鼠每天给予罗氟司特5 mg/kg持续21 d后,PQ诱导的小鼠肺、肝、肾组织损伤与纤维化程度均明显减轻。实验结果表明罗氟司特对PQ诱导的小鼠肺、肝、肾等器官组织损伤与纤维化具有明显的抑制作用。The aim of the present study is to verify whether roflumilast attenuates PQ-induced injury and fibrosis in lung, liver and kidney tissues of mice. Mice are pretreated with PQ of 20 mg/kg （ i. p. ）, and then dealt with roflumilast or dexamethasone 5 rag/ （kg ~ d） for 21 days. The tissues of lung, liver and kidney are surgically separated and immersed in 10% neutral formalin. The injury and fibrosis in tissues of lung, liver and kidney are observed by H＆E staining and Masson staining. The results show that PQ （ 20 mg/kg, i.p. ） induces remarkable toxicity and fibrosis in tissues of lung, liver and kidney. Roflumi- last （5 mg/kg per day for 21 days） treatment reduces PQ-induced injury and fibrosis in tissues of lung, liver and kidney, which is superior to dexamethasone. So, roflumilast attenuates PQ-induced injury and fibrosis in tissues of lung, liver and kidney of mice.

关 键 词：罗氟司特 组织损伤 纤维化 百草枯 小鼠 

分 类 号：Q89[生物学]