耳尖刺血对去卵巢拟阴虚内热大鼠性激素的影响  

Effects of ear-apex bloodletting method on sex hormones in climacteric rat model with interior heat syndrome due to deficiency of Yin

作　　者：王旭[1] 赵百孝[2] 周冰[3] 

机构地区：[1]卫生部中日友好医院中医针灸科,北京100029 [2]北京中医药大学针灸推拿学院,北京100029 [3]中国中医科学院广安门医院,北京100053

出　　处：《中华中医药杂志》2013年第12期3516-3519,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点基础研究发展计划(973计划)(No.2009CB522906)~~

摘　　要：目的:通过观察耳尖刺血对去卵巢拟阴虚内热大鼠血清性激素的影响,探讨耳尖刺血对围绝经期潮热机制,为临床耳尖刺血治疗围绝经期潮热提供实验依据。方法:选用健康雌性Wistar大鼠60只,随机分为5组,即正常组、假手术组、模型组、雌激素组、耳尖刺血组,每组12只。模型组、雌激素组、耳尖刺血组行双侧卵巢切除术,假手术组行同样操作,但不切除卵巢。模型组去卵巢术后10d起开始灌服附子干姜液,连续2周。以后每日灌服蒸馏水20mL/kg,连续2周。雌激素组去卵巢术后10d起开始灌服附子干姜液,连续2周。以后每天灌服用蒸馏水配成的0.003mg/mL己烯雌酚溶液,20mL/kg,连续2周。耳尖刺血组去卵巢术后10d起开始灌服附子干姜液,连续2周。以后每天进行耳尖刺血治疗,连续2周。正常组和假手术组与模型组同时开始每日灌服等容积蒸馏水,连续4周。于末次治疗后24h断头取血5mL采用放射免疫法测定血清雌二醇(E2)、促卵泡生成激素(FSH)、促黄体生成激素(LH);摘取大鼠子宫,精密测量重量并计算子宫指数。结果:造模后,模型大鼠血清E2水平降低,与正常组和假手术组有显著性差异(P<0.01),而血清FSH水平升高,有显著性差异(P<0.01),雌激素组和耳尖刺血组经治疗干预后,血清E2水平较模型组升高,血清FSH水平较模型组降低,且有显著性差异(P<0.05),雌激素组和耳尖刺血组之间无明显差异。血清LH水平各组均无显著性差异。结论:耳穴刺血治疗对去卵巢拟阴虚内热大鼠血清性激素具有很好的良性调节作用,可能是其治疗围绝经期综合征潮热的机制之一。且为临床耳尖刺血治疗围绝经期综合征潮热提供了实验依据。Objective： To observe the effects of ear-apex bloodletting method on sex hormones in climacteric rat model with interior heat syndrome due to deficiency of yin, and to study its therapeutic mechanism on hot flashes and to offer experimental evidence for the clinical practice. Methods： 60 female Wistar rats were randomly divided into 5 groups： normal group, sham-operated group, model group, Diethylstibestrol （DES） group and ear-apex bloodletting（EAB） group,12 rats each. Rats in model group, DES group and EAB group were removed with the ovaries while the sham-operated group with the same operations but without removal of the ovaries. From the 10th day of post-operation, rats in the model group were given Fuzi Ganjiang Decoction by oral gavage for 2 weeks, then distilled water once a day for 2 weeks. From the 10th day of post-operation, rats in DES group were given Fuzi Ganjiang Decoction by oral gavage for 2 weeks, then Diethylstibestrol solution（0.003mg/ mL, 20mL/kg） once a day for 2 weeks. From the 10th day of post-operation, rats in the model group were given Fuzi Ganjiang Decoction by oral gavage for 2 weeks, then treated by ear-apex bloodletting treatment for 2 weeks. The normal group, and sham- operated group were given distilled water by oral gavage, once a day for 4 weeks. After 24h of the last treatment, serum estrogen （E2）, follicle stimulating hormone （FSH）, Luteinizing hormone （LH） contents were detected by radioimmunoassay method. Rat uterus was precisely measured after removal and then for calculation of uterus index. Results： E2 in model group decreased obviously than those of normal group and sham-operated group （P〈0.01） while FSH increased obviously （P〈0.01）. Compared with model group, E2 in DES group and EAB group increased obviously while FSH decreased obviously （P〈0.05）. There was no significant difference among 5 groups in serum LH. Conclusion： Ear-apex bloodletting had benign regulation to serum sex hormones in climacteric rat model with inte

关 键 词：耳尖刺血 围绝经期综合征 潮热 雌二醇 促卵泡生成激素 促黄体生成激素 

分 类 号：R245[医药卫生—针灸推拿学]