平肺口服液对Lewis肺癌小鼠PPAR-γ蛋白和脂联素表达的上调作用  

Effects of Pingfei Mixture on PPAR-γ, adiponectin in Lewis lung cancer mice

作　　者：朱世杰[1] 许灿龙[2] 

机构地区：[1]中日友好医院,北京100029 [2]空军总医院,北京100142

出　　处：《中华中医药杂志》2013年第12期3536-3538,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助项目(No.30973853)~~

摘　　要：目的:探讨平肺口服液对Lewis肺癌小鼠肿瘤组织生长、血清脂联素、瘤组织过氧化物酶体增殖物激活受体γ(PPAR-γ)和Ki-67蛋白表达的影响及抗癌机制。方法:24只Lewis肺癌荷瘤C57小鼠按体质量随机分为正常对照组、模型组、顺铂组和顺铂+平肺组共4组。给药结束后次日取材,测量肿瘤组织重量,采用双抗体夹心ABCELISA法测血清脂联素,用免疫组化法染色检测PPAR-γ和Ki-67蛋白表达。结果:顺铂+平肺组瘤组织重量明显低于模型组(P<0.01),瘤组织PPAR-γ阳性表达分别高于顺铂组及模型组(P<0.01),其血清脂联素水平也高于其他实验组(P<0.01),且瘤组织Ki-67的表达较模型组相比亦明显减少(P<0.01)。结论:平肺口服液可以通过上调Lewis肺癌小鼠肿瘤组织PPAR-γ和血清脂联素的表达起到抑制肿瘤组织生长的作用。Objective： To explore the mechanism of the treatment of lung cancer of Pingfei Mixture. Methods： Use the Lewis lung cancer model of tumor-bearing mice. 24 C57 mice were randomly divided into four groups： normal control group, model group, cisplatin group and cisplatin＋Pingfei groups. Using double antibody sandwich ABC-ELISA method to detect adiponectin. Using immunohistochemical staining to detect PPAR-y and Ki-67 protein expression. Results： The weight of the turner in cisplatio ＋ Pingfei group is lower them model group （P〈0.01）. PPARy expression of cisplatin ＋ Pingfei group is higher than cisplatin group and model group （P〈0.01）, and its serum adiponectin levels are also higher than other experimental groups （P〈0.01）, Ki-67 expression is less than model group （P〈0.01）. Conclusion： Pingfei mixture can raise the expression of PAR-γ and adiponectin to play a treatment effect.

关 键 词：平肺口服液 肺癌 过氧化物酶体增殖物激活受体Γ 脂联素 

分 类 号：R285.5[医药卫生—中药学]