姜黄素对哮喘气道重塑大鼠尾加压素Ⅱ和TGF-β_1表达的调控  

Effects of curcumin on UⅡ and TGF-β_1 in asthmatic airway remodeling rats

作　　者：梁亚峰[1] 张维溪[1] 李昌崇[1] 

机构地区：[1]温州医科大学附属育英儿童医院,温州325027

出　　处：《中华中医药杂志》2013年第12期3539-3542,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(N o.81100015;N o.30571981);浙江省教育厅基金项目(No.Y20070906)~~

摘　　要：目的:研究姜黄素对哮喘气道重塑大鼠肺组织中尾加压素Ⅱ(UⅡ)和转化生长因子-β1(TGF-β1)表达的调控作用。方法:以卵清白蛋白(OVA)致敏与激发建立哮喘气道重塑大鼠模型,24只雄性SD大鼠随机分为对照组、哮喘组和姜黄素组,每组8只。图像分析技术测量大鼠支气管壁总面积和平滑肌面积,计算单位基底膜周径(Pbm)的支气管壁厚度(Wat)和平滑肌厚度(Wam),采用免疫组织化学法测定UⅡ、TGF-β1蛋白表达和逆转录-聚合酶链反应(RT-PCR)检测肺组织UⅡmRNA、TGF-β1mRNA含量。结果:姜黄素组Wat及Wam较哮喘组明显降低(P<0.01),与对照组比较仍有升高(P<0.01);免疫组织化学法显示:姜黄素组肺组织中UⅡ、TGF-β1蛋白表达与哮喘组比较明显减少(P<0.01),仍高于对照组(P<0.01);RT-PCR法显示:姜黄素组肺组织UⅡmRNA、TGF-β1mRNA含量较哮喘组明显降低(P<0.01),仍高于对照组(P<0.01)。结论:姜黄素可以减缓或抑制哮喘气道重塑,降低UⅡ和TGF-β1的表达。Objective： To investigate the effects of curcumin on expressions of UⅡ and TGF-β1 in airway remodeling in asthmatic rats. Methods： Rats were sensitized and challenged by OVA to establish asthmatic model. 24 male Sprague-Dawley （SD） rats were randomly divided into three groups： control group, asthmatic group and curcumin group, with eight rats in each group. The total brochial wall thickness （Wat） and the airway smooth musle thickness （Warn） were measured by image analysis system. The U Ⅱ and TGF-β1 expressions were determined by immunohistochemistry; the UⅡ mRNA and TGF-β1 mRNA contents were detected by RT-PCR. Results： Wat and Wam in the curcumin groups were significantly thiner than those of the asthmatic groups （P〈0.01）, but they were thicker compared with the control groups （P〈0.01）. The UⅡ and TGF-β1 expressions in the curcumin groups significantly decreased than those of the asthmatic groups （P〈0.01）, but they increased compared with the control groups （P〈0.01）. The UⅡ mRNA and TGF-β1 mRNA contents in the curcumin groups were significantly lower than those of in the asthmatic group （P〈0.01）, but they were higher compared with the control groups （P〈0.01）. Conclusion： Curcumin could effectively alleviate the airway remodeling, and inhibit the expressions of UⅡ and TGF-β1.

关 键 词：尾加压素Ⅱ 转化生长因子-Β1 大鼠 哮喘 姜黄素 

分 类 号：R285.5[医药卫生—中药学]