扶正消癌合剂诱导人肺腺癌A549细胞凋亡研究  

Apoptosis inducing effects of Fuzheng Xiaoai Mixture on human lung adenocarcinoma A549 cells

作　　者：杜纪英[1] 古兆森[1] 王淑玲[2] 邓晓明[1] 

机构地区：[1]郑州大学第一附属医院中西医结合科,郑州450052 [2]郑州大学基础医学院病理生理学科,郑州450001

出　　处：《中华中医药杂志》2013年第12期3697-3700,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：河南省科技厅科技攻关项目(No.112300410049)~~

摘　　要：目的:研究扶正消癌合剂对人肺腺癌A549细胞(以下简称A549细胞)凋亡的影响。方法:制备大鼠含药血清,MTT法检测扶正消癌合剂含药血清对A549细胞增值的影响。Annexin V-FITC/PI双染流式细胞术观察含药血清对A549细胞凋亡的影响。Western Blot检测含药血清对P53,B淋巴细胞瘤-2基因(Bcl-2)及Survivin蛋白表达的影响。结果:扶正消癌合剂以时间、剂量依赖方式抑制A549细胞增殖,与DDP合用有协同增效作用,48、72、96h联合组的抑制率分别为28.27%、38.68%、37.98%;其可以诱导细胞的凋亡,且以晚期凋亡为主,凋亡率与对照组比较差异有统计学意义(P<0.05);其在诱导细胞凋亡过程中,下调Bcl-2、Survivin蛋白表达,同时活化P53蛋白表达。结论:扶正消癌合剂可能通过抑制Bcl-2、Survivin蛋白表达,同时活化P53蛋白表达诱导A549凋亡。Objective： To study the apoptosis inducing effects of Fuzheng Xiaoai Mixture on human lung adenocarcinoma A549 cells. Methods： Fuzheng Xiaoai Mixture containing rat serum was prepared and applied on A549. The cell growth inhibition was detected by MTT assay; the apoptosis was detected by Annexin V-FITC/PI staining with flow cytometry. The expression of P53, Bcl-2 and Survivin were analyzed by Western Blot. Results： Fuzheug Xiaoai Mixture could inhibit the proliferation of A549 in the time and dose-dependent manner and had synergies combined with DDP. The inhibitory rate was 28.27%, 38.68% and 37.98% respectively for 48h, 72h and 96h. The herb serum could induce apoptosis, later stage apoptosis accounted main part. Apoptosis rate in the treatment group was higher than that of the control group （P〈0.05）. The herb serum could down-regulate the expression of Bcl-2, Survivin protein, while activation of the P53 protein in the process of induction of apoptosis. Conclusion： Fuzheng Xiaoai Mixture can probably induce the apoptosis of A549 by inhibiting the expression of Bcl-2, Survivin protein, while activation of the P53 protein.

关 键 词：扶正消癌合剂 肺癌 A549细胞 凋亡 P53 B淋巴细胞瘤-2基因 SURVIVIN蛋白 

分 类 号：R285.5[医药卫生—中药学]