镉损伤肝细胞体外模型的建立  

Establishment of the Cadmium-induced Hepatocyte Damage Model

作　　者：杨建泉[1] 倪慧[2] 王玲玲[2] 韩涛[2] 刘学忠[2] 

机构地区：[1]江苏省南通市如东县畜牧兽医站,江苏南通226400 [2]扬州大学兽医学院

出　　处：《现代农业科技》2013年第22期241-243,245,共4页Modern Agricultural Science and Technology

基　　金：江苏省大学生实践创新训练计划(2012JSSPITP1349);国家大学生创新创业训练计划(201211117037)

摘　　要：在建立大鼠原代肝细胞体外培养的基础上,研究了不同浓度的醋酸镉对肝细胞存活率的影响。在原代肝细胞体外培养的不同时间点(4、7、10、24 h),用不同浓度的醋酸镉(0、0.5、1.0、2.0、4.0μmol/L)处理3 h,MTT法测定细胞的相对存活率。结果显示,随着镉剂量的增加,细胞的相对存活率降低。当醋酸镉浓度为4.0μmol/L,细胞的相对存活率为78.35%,与对照组有极显著性差异(P<0.01);用4.0μmol/L醋酸镉在不同时间点处理肝细胞,与对照组相比,细胞存活率均极显著降低(P<0.01)。表明镉损害肝细胞具有一定的浓度依赖性。In this study,the rat primary hepatocyte culture system in vitro was used to study the changes of cell viability in different concentrations of cadmium. In primary hepatocyte cultured at different time points in vitro （ 4 h, 7 h, 10 h and 24 h ）, which were incubated with cadmium acetate of different concentrations （ 0 p, mol/L, 0.5 μmol/L, 1.0μmol/L, 2.0 μmol/L, 4.0 μmol/L） for 3 h.The results of M＇FI＂ assay showed that Cd reduced the motility rat of primary hepatocytes at dose-dependant. After exposed to 4.0 ~mol/L cadmium,the cell motility decreased to 78.35%, showing a significantly difference from the control group（P〈0.01 ）.Hepatocytes were cultured for 4 h, 7 h and 24 h and treated with 4.0 μmol/L Cd for 3 h in vitro, cell viability was significantly decreased （P〈0.01）. The results suggested that Cd-induced cell viability was at a concentration- dependent manner,and the cell initial state could significantly affect Cd-induced cell viability.

关 键 词：镉 肝细胞 大鼠 

分 类 号：O612.6[理学—无机化学]