高效液相色谱-蒸发光散射法测定益母草颗粒中盐酸水苏碱的含量  

Determination of Stachydrine Hydrochloride in Herba Leonuri Granules by HPLC-ELSD

作　　者：黄园 梁月钊 陈晓军 

机构地区：[1]广西博科药业有限公司,广西南宁530007

出　　处：《中医学报》2013年第12期1854-1856,共3页Acta Chinese Medicine

基　　金：基金项目:中华人民共和国专利(编号:200910113825.3)

摘　　要：目的：建立高效液相色谱-蒸发光散射（HPLC—ELSD）测定益母草颗粒中盐酸水苏碱的含量。方法：采用HPLC-ELSD法，安捷伦ZORBAXSB—Aq（4．6mm×250．0mm，5μm）色谱柱；乙腈-水（5：95）为流动相；流速0．3mL·min-1。结果：盐酸水苏碱在0．4004—4．0040μg范围内与峰面积呈良好线性关系，r=0．9992，盐酸水苏碱平均加样回收率为97．11％，RSD为1．11％，精密度、稳定性良好。结论：本方法可靠性高，分离度、准确性、重复性好，适用于益母草颗粒中盐酸水苏碱的含量测定。Objective ： To establish a method for determination of Stachydrine Hydrochloride in Herba Leonuri Granules. Methods ： Evapo- rative light scattering detecctor （ELSD）, and an Agilent ZORBAX SB-Aq （4.6 mm × 250.0 mm, 5 μm） chromatographic column were used for. The mobile phase was a mixture of acetonitrile-water（5 ： 95 ）, the flow-rate was 0.3 mL · min-l. Results：The calibration curve of Stachydrine Hydrochloride was linear over the range of 0. 4004 - 4.004 μg（ r = 0. 999 2）. The average recovery of Stachydrine Hydrochloride was 97.11%, and RSD 1.11%. The precision and stability were fine. Conclusion ： HPLC-ELSD may be used in the quan- titative analysis of Stachydrine Hydrochloride in Herba Leonuri Granules with high reliability, separation, accuracy, repeatability.

关 键 词：高效液相色谱-蒸发光散射法 益母草颗粒 盐酸水苏碱 

分 类 号：R284.1[医药卫生—中药学]