含乌骨藤散质量标准研究  

Quality Standard Research on Glaucescent Fissistigma Root Powder

作　　者：李广胜[1] 

机构地区：[1]河南中医学院第三附属医院,河南郑州450008

出　　处：《中医学报》2013年第12期1873-1874,共2页Acta Chinese Medicine

基　　金：河南省自然科学基金项目(编号:0641130502)

摘　　要：目的：建立含乌骨藤散的质量标准。方法：采用试管反应和TLC对方中乌骨藤进行定性鉴别，采用反相HPLC对方中乌骨藤所含的绿原酸进行含量测定。结果：试管反应良好，沉淀明显，薄层色谱斑点明显。绿原酸在0．1548～1．0320μg时，峰面积对进样量呈良好的线性关系，r=0．99995（n=6），平均回收率为97．45％，RSD为1．24％（n=6）。结论：所建立的方法简便、准确、重复性好，可作为含乌骨藤散的质量控制标准。Objective：To establish the quality standard for Glancescent Fissistigma Root Powder. Methods：The qualitative identification of Glaucescent Fissistigma Root was carried out by using in-vitro reaction and TLC and the content determination of chlorogenic acid in Glaucescent Fissistigma Root was carried out by using RP-HPLC. Results：In-vitro response was good, the precipitation was obvious and TLC spots were clear. Peak area of sample volume had good linear relationship when the contents of chlorogenic acid was in the range of 0. 154 8 - 1. 032 0μg （ r = 0.999 95, n = 6）. The average recovery rate was 97.45 % , RSD was 1.24% （ n = 6）. Conclusion ： The estab- lished method is simple, accurate and reproducible,which can be used as quality control standards of Glancescent Fissistigma Root.

关 键 词：含乌骨藤散 反相HPLC 质量标准 

分 类 号：R285.2[医药卫生—中药学]