p21在电离辐射诱导EL-4细胞G_1期阻滞中的作用  

The role of p21 in G_1 arrest of EL-4 cells induced by ionizing radiation

作　　者：傅海青[1] 鞠桂芝[1] 罗灿[1] 傅士波[1] 

机构地区：[1]白求恩医科大学卫生部放射生物学重点实验室,长春130021

出　　处：《中华放射医学与防护杂志》2000年第6期385-387,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目!( 3 9770 193 )

摘　　要：目的　探讨 p2 1在电离辐射诱导EL 4细胞G1期阻滞中的作用。方法　采用North ernblot检测 p2 1WAF1mRNA水平的变化 ;采用流式细胞术检测 p2 1蛋白表达及细胞周期的变化。结果　 4 0GyX射线照射后 12h、2 4h、48hG1期EL 4细胞百分数明显高于假照射组 ;p2 1WAF1mRNA水平从照射后 1h开始升高 ,4h达峰值 ,持续至照后 12h ;p2 1蛋白表达在照射后 2～ 48h明显增高。结论　 4 0GyX射线照射可诱导EL 4细胞G1期阻滞 ,p2 1在电离辐射诱导EL 4细胞G1期阻滞中起重要作用。Objective To elucidate the role of p21 in G 1 arrest of EL-4 cells induced by ionizing radiation. Methods\ Northern blot was used to measure p21 WAF1 mRNA level.Flow cytometry was employed to analyze p21 protein expression and cell cycle of EL\|4 cells. Results\ Following 4\^0 Gy X\|irradiation,EL\|4 cells in G 1 phase began to significantly increase at 12 h,reached the peak at 24 h,and kept at high level till 48 h;the p21 WAF1 mRNA level began to increase at 1 h,reached the peak level at 4 h,kept the high levels till 12 h,then recovered to normal at 24 h;and the level of p21 protein expression in EI\|4 cells increased significantly at 2 h to 48 h. Conclusion\ The results suggest that p21 might play an important role in G 1 arrest of EL\|4 cells induced by ionizing radiation.\;

关 键 词：电离辐射 G1期阻滞 P21 EL-4细胞 

分 类 号：Q691.5[生物学—生物物理学] R811.5[医药卫生—放射医学]