早期胃内灌注ω-3多不饱和脂肪酸对失血性休克大鼠肠黏膜屏障功能影响的实验研究  

Experimental study on influence of early intragastric infusionω-3 PUFAs on intestinal barrier function of hemorrhagic shock in rats

作　　者：郭佳钰[1] 王湘[1] 黄云[1] 周娟[1] 

机构地区：[1]中国人民解放军第三军医大学护理学院

出　　处：《护理研究（上旬版）》2013年第12期3865-3867,共3页Chinese Nursing Researsh

基　　金：2012年国家级大学生创新创业计划项目;编号:201290035033

摘　　要：[目的]探讨失血性休克复苏后早期胃内灌注ω-3多不饱和脂肪酸(ω-3PUFAs)对大鼠肠黏膜屏障功能的影响。[方法]选取健康成年SD大鼠72只,按照胃内灌注状况,随机分为ω-3PUFAs组、牛奶组、不干预组。乌拉坦腹腔麻醉后,制作休克大鼠模型,休克30 min后复苏,ω-3PUFAs组和牛奶组在复苏结束、复苏2h分别进行胃内灌注。各组分别在休克30min、复苏2h和4h处死1/3大鼠,取肝脏组织进行细菌培养,取一段空肠制成病理切片。[结果]肝脏组织细菌培养显示,ω-3PUFAs组、牛奶组复苏2h、4h菌落数较休克30min少,同时限点也较不干预组少(P<0.05);肠道病理切片显示,复苏2h、4hω-3PUFAs组较牛奶组和不干预组轻,不干预组损伤最重。[结论]失血性休克救护过程中,早期胃内灌注ω-3PUFAs更有利于肠道屏障功能的恢复、减少肠内细菌移位。Abstract Objective：To probe into influence of early intragastric perfusion of ω- 3 PUFAs in hemorrhagic shock rats after resuscitation on intestinal barrier function of hemorrhagic shock in rats. Methods： Sprague - Dawley rats were randomly divided into 3 group in accordance with the intragastric perfusion：ω- 3PUFAs group.milk group and the control group. After in- traperitoneal urethane anesthesia, shock rats models were produced, resus- citation was carried out for them after shock for 30 min. The models in o^- 3PUFAs group, milk group received intragastrical infusion respectively at the end of resuscitation and at two hours after resuscitation. Each 1/3 of rats in each group were killed at 30 minutes after shock and at 2 and 4 hours after resuscitation, separately. The liver tissue was taken for bacterial culture and a segment of jejunal was taken to make pathological section. Results：Liver tissue cultures showed that,the number of colonies in ω - 3 PUFAs group and milk group at 2 and 4 hours after resuscitation was less than that at 30 min after shock, and was less than that in non -intervention group at the same time （P〈0.05） ;intestinal biopsy showed that the dam- age of ω - 3 PUFAs group was lighter than that of milk group and non - in- tervention group at 2 and 4 hours after resuscitation, the damage was the most serious in non - intervention group. Conclusion：During ambulance of hemorrhagic shock,early intragastric infusion of ω- 3PUFAs is more con- ducive to the recovery of intestinal barrier function and reducing intestinal bacterial translocation.

关 键 词：大鼠 失血性休克 Ω-3多不饱和脂肪酸 胃内灌注 肠黏膜屏障 细菌移位 

分 类 号：R459.7[医药卫生—急诊医学]