雌激素对体外培养破骨细胞功能的影响  

Effects of estrogen on the cultured osteoclasts in vitro

作　　者：鲁道海[1] 张忠民[2] 陈映红[1] 陈建庭 金大地 郑晓明[1] 

机构地区：[1]解放军第一六一医院骨科,武汉市430010 [2]第一军医大学附属南方医院骨科国家骨质疏松诊疗研究中心

出　　处：《中华老年医学杂志》2000年第6期431-434,共4页Chinese Journal of Geriatrics

基　　金：承默沙东国际交流基金

摘　　要：目的　探讨雌激素对体外培养破骨细胞功能的影响。　方法　利用酶动力学法测定培养基中酸性磷酸酶 (ACP)和抗酒石酸酸性磷酸酶 (TRAP)活性 ;共聚焦显微镜观察破骨细胞内氢离子随雌激素浓度的变化情况 ;LeicaQuantimet 5 0 0图像分析系统对骨吸收陷窝进行图像分析 ;扫描电镜观察骨吸收陷窝变化。　结果　随着雌激素浓度的升高 ,处理组与对照组ACP和TRAP的活性分别从(1 6 9± 0 13)U/L和 (1 6 0± 0 14)U/L降低到 (1 16± 0 31)U/L和 (0 93± 0 34)U/L(均为P <0 0 1) ,骨吸收陷窝的数目与面积从 (13 2 5± 1 5 2 )个 /片和 (4 82 2 7± 2 2 5 2 3) μm2 减少到 (4 6 8± 0 2 4)个 /片与(35 6 35± 145 41) μm2 ,处理组与对照组比较差异具有显著性意义 (P <0 0 1)。破骨细胞相对荧光值经过短暂的下降后持续上升 ,在第 12 0 0s时达到最高值。　结论　雌激素能够抑制氢离子释放 ,同时能改变ACP和TRAP活性 ,因而能直接抑制破骨细胞的骨吸收功能。Objective To study the effects of estrogen on the cultured osteoclasts in vitro. Methods The acid phosphatase (ACP) and tartrate resistant acid phosphatase (TRAP) activities were measured kinetically and the introcellular hydrogen ions were calculated with the confocal laser scanning microscopy(CLSM). The area and number of the resorption pits were determined with the Leica Quantimet 500 system. Bone resorption pits were observed with scanning electronicmicroscope. Results With the estrogen concentration increased, the ACP and TRAP activities decreased from (1 69±0 13) U/L and (1 60±0 14) U/L to (1 16±0 31) U/L and (0 93±0 34) U/L, respectively (experiment group vs control group, P <0 01) In addition, the area and number of the resorption pits reduced from (13 25±1 52) and (482 27±225 23)μm 2 to (4 68±0 24) and (356 35±145 41)μm 2 , respectively ( P <0 01). Conclusions Estrogen could not only inhibit hydrogen ions yielding, but also change ACP and TRAP activities, therefore inhibit the bone resorption by osteoclastes.

关 键 词：雌激素 破骨细胞 骨质吸收 体外培养 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学]