主动脉中膜细胞体外钙化的研究  

A study on calcification of cultured aortic medial cells in vitro

作　　者：崔晓暄[1] 王士雯[2] 齐鹏[2] 

机构地区：[1]北京市空军总医院老年科,100036 [2]解放军总医院老年心血管病研究所

出　　处：《中华老年医学杂志》2000年第6期447-449,共3页Chinese Journal of Geriatrics

基　　金：国家自然科学基金!(39770 86 9)

摘　　要：目的　研究主动脉中膜细胞体外钙化情况以及 2 5 羟基胆固醇、β 甘油磷酸盐对此过程的作用。　方法　外植块法分离培养主动脉中膜细胞 ,进行vonKossa染色以观察钙化情况 ,生化检测细胞内外不溶性钙 ,放免法测定培养基质骨钙素含量。　结果　原代细胞有两种生长表现 :一种细胞平行生长 ,不形成结节 ,vonKossa染色阴性 ;一种易形成结节 ,vonKossa染色阳性。传代细胞 (对照组 )培养 2 8d无结节形成 ,vonKossa染色阴性 ,但是 ,2 5 羟基胆固醇 (胆固醇组 )、β 甘油磷酸盐 (甘油磷酸盐组 )分别刺激有细胞结节形成 ,vonKossa染色阳性 ,不溶性钙分别为 (5 7 80± 18 5 0 ) μg/孔、(6 7 5 0± 15 30 ) μg/孔 ,培养上清骨钙素分别为 (0 886± 0 0 6 3) μg/L、(0 895± 0 0 6 1) μg/L ,与对照组比较 ,差异有显著性。　结论　体外培养的主动脉中膜细胞有两种亚型 ,一种为平滑肌细胞的表型 ,另一种与微血管周细胞相似 ,并能使其细胞外基质钙化。 2 5 羟基胆固醇及 β 甘油磷酸盐均可以促进这一体外钙化过程。动脉中膜体外钙化过程中骨钙素合成增多 ,可能参与了主动脉的钙化。Objective To investigate calcification of cultured aortic medial cells in vitro and acceleration by 25 hydroxycholesterol or β glycerophosphate. Methods Aortic medial cells were obtained by explantation, and the calcification was observed by von Kossa staining. Insoluble calcium precipitation in cellular layer was determined by biochemical method , and osteocalcin in the media was analyzed with radioimmunoassay. Results Two different types of primary cells were shown from culture: one with parallel cellular growth and being negative by von Kossa staining, the other cell type formed cellular nodules with positive von Kossa staining. After 28 days of cell passages, cell growth appeared no nodule formation. However, many cellular nodules and positive von Kossa staining were observed in the passaged cells treated with 25 hydroxycholesterol or β glycerophosphate, and both insoluble calcium 〔(57 80±18 50)μg/pool, (67 50±15 30)μg/pool〕and osteocalcin 〔(0 886±0 063)μg/L, (0 895±0 061)μg/L〕in the medium were significantly increased than that of the untreated cells. Conclusions Cultured aortic medial cells could be divided into two subtypes, one with the characters of smooth muscle cells, the other with the micro vascular pericytes which could calcify the extracellular matrix. 25 hydroxycholesterol and β glycerophosphate promoted the in vitro calcification, and osteocalcin secretion was increased in the process of calcification of aortic medial, suggesting that osteocalcin might participate in the aortic calcification.

关 键 词：主动脉中膜细胞 钙化 胆固醇 甘油磷酸 血管钙化 

分 类 号：R543.02[医药卫生—心血管疾病]