小鼠甲醇氧化应激的电子顺磁共振的研究  

Methanol-Induced Oxidative Stress in Mice Studied by Electron Paramagnetic Resonance

作　　者：周丽芳[1] 朱翔[1] 赵红莉[1] 蓝闽波[1,2] 史新梅[2] 

机构地区：[1]华东理工大学,上海市功能性材料化学重点实验室,上海200237 [2]华东理工大学,分析测试中心,上海200237

出　　处：《波谱学杂志》2013年第4期507-515,共9页Chinese Journal of Magnetic Resonance

基　　金：上海市科委纳米专项资助项目(12nm0501100)

摘　　要：建立了小鼠甲醇中毒的氧化应激模型,研究了甲醇诱导小鼠产生氧化应激状态,探讨了大黄中草药和维生素C对小鼠甲醇氧化应激的保护作用.模型小鼠共分为5个组:空白组、对照组、甲醇应激组、大黄组、维生素C(Vc)组.利用电子自旋捕捉技术研究了小鼠甲醇氧化应激不同模型组小鼠体内的肝、肾、脾、心、肺和脑中产生的自由基强度的变化.结果表明:甲醇诱发了小鼠体内自由基的产生,甲醇组和生理盐水组相比,自由基的强度明显增强(显著性差异统计指标P<0.01);而大黄和Vc对小鼠甲醇氧化应激有保护作用,大黄组、Vc组和甲醇组相比自由基的强度则明显降低(P<0.01).A mouse model of acute methanol poisoning was established to evaluate the protective effects of rhubarb and vitamin C （Vc） on methanol-induced oxidative stress in different organs.The mice used in the study were randomly divided into five group：blank group,control group,methanol group,rhubarb group and Vc group.EPR spin trapping technique was employed to study the intensity change of spin adducts induced by reactive oxygen species （ROS） free radicals generated in different organs of the mice.Compared with the control group,the animals in the methanol group showed significantly increased amount of ROS free radicals in the organs,suggesting the induction of acute oxidative stress.Rhubarb and Vc were shown to be able to alleviate acute methanol-induced oxidative stress.

关 键 词：电子顺磁共振(EPR) 氧化应激 自旋捕捉 小鼠 甲醇 自由基 

分 类 号：O657.61[理学—分析化学]