超滤法结合液质联用法测定姜黄素大鼠血浆蛋白结合率  

Determination of Plasma Protein Binding Rates of curcumin by Ultrafiltra-tion Coupled with LC-MS

作　　者：黄秀旺[1] 杨蕾[1] 邓艳平[1] 吴国华[1] 许建华[1] 

机构地区：[1]福建医科大学药学院,福州350001

出　　处：《海峡药学》2013年第11期43-46,共4页Strait Pharmaceutical Journal

基　　金：福建省自然科学基金资助项目(2010J01180);福建省卫生厅中医药科研课题(WZZY0904);福建医科大学重大科研项目计划(09ZD006)

摘　　要：目的建立液相色谱串联质谱（LC-MS）测定方法并检测姜黄素的大鼠血浆蛋白结合率。方法建立LC-MS检测方法，结合超滤法测定姜黄素的血浆蛋白结合率。结果3种浓度（100、300和1000ng·mL^-1）姜黄素与大鼠血浆蛋白结合率分别为（80．3±5．28）％、（75．3±4．45）％和（62．64—1．98）％。结论姜黄素血浆蛋白结合率较高，LC—MS可用于姜黄素血浆蛋白结合率的测定。OBJECTIVE To establish the method with liquid chromatography-mass spectrophotometer（LC-MS） and determine the protein binding rates of curcumin in plasma of rat. METHODS The ultrafiltration combined with LC-MS was performed to determine the drug concentration of curcumin, and its protein binding rates in rat. RESULTS With different concentration of curcumin including 100,300 and 1000ng ~ mL-1 , the protein bind- ing rates of curcumin in rat were （ 80. 3 ± 5.28 ） %, （ 75.31± 4, 45 ） % and （62. 6 ± 1.98 ） %. CONCLUSION curcumin has higher level in binding to plasma protein of rat. The LC-MS method can be used to determine the drug concentration of curcumin properly.

关 键 词：姜黄素 血浆蛋白结合率 超滤法 液相色谱串联质谱(LC-MS) 

分 类 号：R284.1[医药卫生—中药学] R965[医药卫生—中医学]