影响绵羊胞质内单精子注射结果相应因素的分析  

Analysis of the Relevant Factors Affecting the Intracytoplasmic Sperm Injection in Sheep

作　　者：朴学娇[1] 郑雪莹[1] 曹玉桃[1] 张帆[1] 翟春东 刘云海[1] 倪和民[1] 郭勇[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《中国农学通报》2013年第32期34-38,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然基金面上项目"不同来源植入前期绵羊胚胎IFNτ差异表达及其体外诱导子宫上皮细胞Galectin15表达的研究"(31072185);国家自然基金面上项目"IFNτ协调Galectin 15对绵羊体外胚胎着床调节机制的研究"(30871836)

摘　　要：本实验探讨了绵羊卵母细胞成熟以及胞质内单精子注射的相应影响因素,以期优化绵羊ICSI的操作技术和体外成熟体系。本研究分别设定3组卵母细胞成熟时间（19~21 h,23~25 h,28~30 h）,同时比较6、12点和7、11点注射位置对胞质内单精子注射后胚发育的影响。结果表明,绵羊卵母细胞在成熟23~25 h之间进行单精子注射后,其囊胚率显著高于19~21 h和28~30 h[（6.30±0.59）%vs（4.05±0.63）%,（3.59±0.29）%],研究注射位置对胚胎发育影响时研究结果显示,在6、12点注射后其胚胎的卵裂率（54.17±3.24）%vs（46.40±3.22）%和囊胚率（5.88±0.30）%vs（2.77±0.15）%均明显高于7、11点。因此,应尽量使用成熟23~25 h的绵羊卵母细胞进行胞质内单精子注射,同时注射时应选择6、12点的位置,从而利于胚胎的后期发育。Through investigating the effects of the position morphology of the first polar body in sheep oocyte and the time of oocyte maturation in intracytoplasmic sperm injection （ICSI）, the operation of sheep ICSI technology and in vitro maturation system was designed to optimize. It set three groups in accordance with the maturation time （19-21 h, 23-25 h, 28-30 b） while the first polar body located at 6 o＂ clock or 12 or clock were compared with those at 7 o＇clock or 11 o＇clock in terms of their effect. The results displayed that blastocyst rate of ooeyte that sperm injection in mature between 23-25h was significantly higher than that between 19-21 h and 28-30 h （6.30±0.59）% vs （4.05±0.63）%, （3.59±0.29）%, whereas the first polar body located at 6 o＂ clock or 12o＂ clock had a higher cleavage embryo and blastocyst rate than that located at 7 or clock or 11 o＂ clock. Therefore, the experiments showed that the first polar body located at 6 or clock or 12o＂ clock, and the 23-25 h maturation time, was conducive to the normal fertilization and embryo development.

关 键 词：体外成熟 胞浆内单精子注射 极体位置 胚胎发育 

分 类 号：S814[农业科学—畜牧学]