紫衫醇对小鼠不同组织巨噬细胞功能的影响  

Effects of Paclitaxel on the Functions of Different Tissue Macrophages from Mice

作　　者：陈舒楠[1] 官佳懿[1] 谷崇高 芦山[1] 沈红[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《中国农学通报》2013年第32期49-53,共5页Chinese Agricultural Science Bulletin

基　　金：北京市教育委员会科技面上项目"科研基地-科技创新平台-抗菌中兽药成分组方原则及新兽药研发"(PXM2012_014207_000013);北京市属高等学校人才强教计划资助项目"动物抗炎-促生长中药研究"(PHR201107134)

摘　　要：为了探讨紫杉醇(Paclitaxel,PA)对小鼠不同组织巨噬细胞增殖和功能的影响。无菌操作制备小鼠腹腔、骨髓和脾脏巨噬细胞,在体外细胞培养体系中分别加入不同浓度40、80、160 ng/mL的PA和脂多糖(LPS)共培养,采用MTT法检测细胞增殖,ELISA和Griess法分别检测细胞分泌TNF-α和NO量,瑞氏-吉姆萨染色法测定腹腔巨噬细胞吞噬能力。结果表明:PA促进腹腔巨噬细胞和LPS诱导的腹腔和骨髓巨噬细胞增殖,但抑制脾脏和骨髓巨噬细胞以及LPS诱导的脾脏巨噬细胞增殖;PA抑制不同组织巨噬细胞分泌TNF-α,但促进腹腔和脾脏巨噬细胞分泌NO;PA提高了腹腔巨噬细胞吞噬鸡红细胞的能力。PA对小鼠不同组织巨噬细胞的免疫功能均有影响,这为进一步阐明PA的免疫调节机理提供了新的理论依据。To investigate the effects of Paclitaxel（PA） on proliferation and function of different tissue macrophages separated from mice. Peritoneal, bone marrow and spleen macrophages were prepared by aseptic technique and in vitro cultured in media containing different concentration of 40, 80,160 ng/mL PA alone or respectively together with lipopolysaccharide （LPS）. Cell proliferation was detected by MTY, TNF-ot and NO level were assayed by ELISA and Griess methods respectively. Phagocytic ability of peritoneal macrophages was analyzed by Wright-Giemsa staining method. The results showed that PA could significantly promote peritoneal macrophage and LPS-stimulated peritoneal and bone marrow macrophages proliferation, but inhibit spleen and bone marrow macrophages and LPS-stimulated spleen macrophages. TNF-α secretion of different tissue macrophages was inhibited by PA, but NO secretion of peritoneal and spleen macrophages was promoted by PA. PA enhanced the phagocytosis of peritoneal macrophages to chicken red blood cells. PA had different impacts on the immune functions of different tissue macrophages from mice, which might be provided a new theoretical basis for the mechanism to regulate cellular immune function of PA.

关 键 词：紫杉醇 小鼠 巨噬细胞 免疫功能 

分 类 号：S852.161[农业科学—基础兽医学]