中药复方A对施氏鲟抗氧化能力的影响  

Effects of Prescription A on Anti-oxidation Capability in Amur sturgeon (Acipenser schrencki Brandt.)

作　　者：王荻[1] 刘红柏[1] 

机构地区：[1]中国水产科学研究院/黑龙江水产研究所,哈尔滨150070

出　　处：《中国农学通报》2013年第32期87-90,共4页Chinese Agricultural Science Bulletin

基　　金：公益性行业(农业)科研专项经费"冷水性鱼类病害防治技术研究"(201003055);中央级公益性科研院所基本科研业务费专项"冷水性鱼类主要流行性疾病中药防治技术研究"(201001)

摘　　要：为了研究自组中药方剂(复方A)对施氏鲟组织中抗氧化功能相关的活性物质含量及酶活性的影响及其在高温应激条件下对鲟鱼的免疫调节功能。将1+龄实验鱼随机分为2组,每组设3个平行,每个平行20尾鱼,采用口灌水煎剂的方法,按照1.1 g/kg体质量的剂量每日口灌给药1次,连续给药7天,并设口灌蒸馏水的对照组。实验结束后,取部分鱼高温30℃热刺激2 h后与常温22℃给药的实验组共同采样,对鱼血浆、肝脏及红细胞中SOD活性;血浆及肝脏中MDA含量;血浆及肝脏中NO含量和NOS活性进行了测定。结果表明:复方A能有效调节鲟鱼体内NO含量及NOS活性,显著提高机体SOD活性,并降低MDA含量;在高温应激条件下仍能保持良好的作用效果,保护机体免受氧化损伤。复方A是一种适用于鲟鱼养殖使用的免疫添加剂,在高温应激条件下仍有较好效果。In order to research the enzyme activities and contents of active materials, which were involved in anti-oxidation capability of tissues, were investigated in Amar sturgeon （Acipenser schrencki Brandt.） when oral given the prescription of Traditional Chinese Medicine A. The immune function of prescription A on sturgeons was also studied under high temperature stress condition. 1 age old fish were divided into 2 groups each （n=60 in each group）, with each group subdivide into 3 replicates of 20 fish per tank. A control group, in which distilled water was administered orally, was also tested. Prescription A was orally given to experimental fish at a concentration of 1.1 g/kg body weight continuously for 7 days. After the administration of drugs, parts of fish were stimulated for 2 hours at high temperature （30℃） and samples were collected. Superoxide Dismutase （SOD） activity in plasma, liver and red blood ceils, Malondialdehyde （MDA） and Nitric Oxide （NO） content in plasma and hver, and Nitric Oxide Synthase （NOS） activity in plasma and liver, were measured and compared. The results indicated that prescription A could efficiently regulate NO content and NOS activity, increase SOD activity and decrease MDA content in several tissues of Amur sturgeon. The prescription A also brought better effects to protect the body from oxidative damage under the thermal stimulation. Thus, the prescription A was a suitable Traditional Chinese Medicine for sturgeon farming as an immune additive since it still had fine effect under the high temperature stress condition.

关 键 词：复方A 施氏鲟 抗氧化 影响 

分 类 号：S942.5[农业科学—水产养殖]