花鲈皮肤溃疡病病原菌的分离与鉴定  

Isolation and Identification of the Pathogens of the Skin Ulceration Disease in Lateolabrax japonicus

作　　者：林星 

机构地区：[1]福建莆田市生物工程研究所,福建莆田3511000

出　　处：《中国农学通报》2013年第32期100-104,共5页Chinese Agricultural Science Bulletin

基　　金：福建省科技厅星火计划项目"中国花鲈淡化养殖技术集成与示范"(2011S0015)

摘　　要：为了确定海水池塘养殖花鲈发生皮肤溃疡病的病原菌及其防治方法。利用莆田市人工饲养的花鲈中挑取具有皮肤溃疡病明显症状的幼鱼,从体表病灶和体内组织中分离出病原菌,通过人工感染试验、细菌形态观察及常规生理生化指标测定对其进行了分类学鉴定,同时进行药敏试验。结果表明:引起该养殖场皮肤溃疡病的病原菌为哈维氏弧菌,该菌对复方新诺明、阿莫西林、哌拉西林、替卡西林、头孢拉定、头孢噻肟、头孢吡肟、头孢他啶、头孢呋辛钠、美罗培南、头孢西丁、卡那霉素、奈替米星、阿米卡星等14种药物敏感,对米诺环素、链霉素等抗生素表现出耐药。在实际生产上采用饲料中添加复方新诺明和维生素C的方法来治疗花鲈皮肤溃疡病,连续用药10天后,取得了较好的疗效。In order to find the pathogens of the ulceration disease of Lateolabrax japonicus in seawater pond culture and the prevention and control methods of its diseases and pests, the diseased ulceration diseasal Lateolabrax japonicus were selected from Putian bacterials were isolated from the disease Lateolabrax-japonicus and were confirmed to be the pathogen of the disease by artificial infection test. According to the results, morphological feature, physiological and biochemical characteristics were very similar to VibHo-harveyi. Experiments showed that Aeromonas hydrophila was sensitive to Cotrimoxazole, Amoxieillin, Piperaeillin, Tieareillin, Cefradine, Cefotaxime, Cefepime, Ceftazidime, Cefuroxime sodium, Meropenem, Cefoxitin, Amikaein, Netilmiein, Amikaein and other 14 kinds of antibiotics, but it was insensitive to minoeycline, streptomyein and so on. By adding compound Cotrimoxazole and vitamin C in feed to the treatment of Lateolabrax japonieus skin ulcer disease in the actual produetion, eontinuous administration for 10 days, has obtained the good eurative effect.

关 键 词：花鲈 皮肤溃疡病 分离鉴定 哈维氏弧菌 药敏试验 

分 类 号：S941.42[农业科学—水产养殖]