玉米种质资源亲缘关系的分子标记鉴定及其耐盐性评价  

Identification of Genetic Relationship of Corn Germplasms with Molecular Markers and Evaluation of Their Salt Tolerance

作　　者：冯艳[1] 江鹏[1] 王亚峰[1] 李金贝[1] 吴雪玲[1] 汪保华[1] 李平[1] 

机构地区：[1]南通大学生命科学学院农业部南方平原地区玉米科学观测站,江苏南通226019

出　　处：《中国农学通报》2013年第33期79-84,共6页Chinese Agricultural Science Bulletin

基　　金：江苏省高校"青蓝工程"优秀青年骨干教师培养项目;南通市科技平台建设计划"南通市作物重要基因资源发掘与分子育种重点实验室"(CP12012002);南通大学特聘教授人才经费项目(03080367);南通市农村科技创新及产业化项目"玉米耐盐性的鉴定评价及耐盐分子育种"(HL2013026);南通大学研究生科技创新计划项目"玉米耐盐性状综合评定及耐盐QTL定位研究"(YKC13054)

摘　　要：为鉴定10份玉米材料的亲缘关系,测试其耐盐性强弱并初步探讨玉米耐盐的生理机制,采用20对SSR引物对10份玉米材料的DNA进行PCR扩增,采用NJ法进行聚类分析,构建亲缘关系图;同时分别配置浓度为0、100、200、300 mmol/L的NaCl溶液,对幼苗进行盐胁迫实验,并测定株高作为耐盐性的指标;7次盐处理后,对抗氧化物酶包括超氧化物歧化酶和过氧化物酶活性进行了鉴定。根据分子标记的结果初步鉴定出了10个玉米材料间的亲缘关系,10份材料的遗传相似系数变化范围为0.54～0.71。结合株高和酶活的测定结果筛选出了耐盐性较强的玉米材料5份,分别为‘先玉335’、‘郑单958’、LH214、83IBI4和LH204。研究结果对于玉米耐盐育种的亲本组合选配具有一定意义。In order to analyze the genotypes of 10 corn accessions,detect their salt-tolerant level and reveal salt-tolerance mechanism in corn,20 pairs of simple sequence repeat（SSR） molecular markers were used to amplify the 10 corn accessions,and the corresponding SSR fingerprints were obtained to collect 0,1 data. The method of NJ cluster analysis was performed to construct dendrograms. Meanwhile,NaCl solution with the concentration of 0,100,200 and 300 mmol/L,respectively was prepared and employed on the corn seedlings.Plant height of the corn materials was measured as the reference data of salt resistance. After seven times of treatment with NaCl solution,the activity of antioxidative enzymes including superoxide dismutase（SOD） and peroxidase（POD） was detected. The genetic relationships of the 10 corn accessions were identified according to the molecular marker data,with the genetic similarity coefficient varying from 0.54 to 0.71. Based on the plant height data and the results of enzymatic activity,5 salt-resistant corn materials which were‘XianYu335＇,‘ZhengDan958＇,LH214,83IBI4 and LH204,were chosen. The results would be helpful for choosing the best parents combinations.

关 键 词：玉米 聚类分析 耐盐性 酶活性 株高 

分 类 号：S513[农业科学—作物学]