水杨酸对盐胁迫下唐古特白刺活性氧代谢和细胞膜透性抑制的缓解效应  

Effects of Salicylic Acid Mitigating under Salt Stress on Active Oxygen Metabolism and Membrane Stability in Nitratia tangutorum

作　　者：张艳艳[1] 刘威[1] 宣亚楠 袁晓婷[1] 闫永庆[1] 

机构地区：[1]东北农业大学,哈尔滨150030

出　　处：《东北林业大学学报》2013年第12期56-59,共4页Journal of Northeast Forestry University

基　　金：黑龙江省教育厅科学技术研究项目(11551052);东北农业大学博士启动基金项目(2012RCB63);黑龙江省自然科学基金项目(C2007-16)资助

摘　　要：设置4个浓度(0、0.5、1.0和1.5 mmol/L)的水杨酸处理400 mmol/L NaCl胁迫下的白刺(Nitratia tangutorum),并研究外源水杨酸对盐胁迫下白刺的缓解机制。结果表明:外施不同浓度的水杨酸(SA)后,白刺叶片质膜所受伤害均有不同程度的缓解,其中1.0 mmol/L SA浓度下白刺叶片膜透性变化较小;丙二醛作为膜脂过氧化的产物,其变化规律与膜透性呈显著正相关,白刺叶片中的超氧化物歧化酶、过氧化物酶和过氧化氢酶活性在处理9 d时均达到最高,增强了清除细胞内活性氧的能力;0.5 mmol/L的SA在3 d时能够缓解盐胁迫,但长时间后其缓解作用不明显,1.5 mmol/L SA在一定程度上表现出胁迫作用。综合各项指标,SA浓度在1.0 mmol/L时,可以在第9天缓解400 mmol/L NaCl胁迫对白刺造成的伤害,但随着SA浓度的下降,其缓解作用持续的时间也变短。We treated Nitratia tangutorum for mitigative effect under 400 mmol / L NaCl stress with 0,0. 5,1. 0 and 1. 5 mmol / L salicylic acid（ SA）. Injury to the plasma membrane of Nitratia tangutorum,which was induced by salinity of 400 mmol / L,decreases with different contents of SA,keeps stable under 1. 0 mmol / L SA. The malondialdehyde is served as plasmalemma peroxide product,and its variation is consistent with that of electrolyte leakage with a significant positive correlation（ r=0. 439）. The activities of superoxide dismutase,peroxidase and catalase are the highest after nine-day treatment,and they can enhance the ability of clearing the reactive oxygen. The mitigative effect is obvious under 0. 5 mmol / L SA in three days,but is not obvious for a long time treatment. 1. 5 mmol / L SA is a bad performance to Nitratia tangutorum. Therefore,the tolerance of Nitratia tangutorum to complex saline stress is when the SA is 1. 0 mmol / L in nine days,and the less the content of SA is,the shorter the time of mitigative effect is.

关 键 词：白刺 水杨酸 保护酶活性 

分 类 号：S688.9[农业科学—观赏园艺]