贞栀颗粒中女贞子提取效率的影响因素考察及醇提工艺优选  

Investigation of Factors Influencing on Extraction Efficiency of Active Constituents in Ligustri Lucidi Fructus and Optimization of Ethanol Extraction Process of Zhenzhi Granules

作　　者：王莹[1,2] 曹伟[1,2] 贺俊杰[2] 陈彦[1,2] 朱方石 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]江苏省中医药研究院中药新型给药系统重点实验室,国家中医药管理局中药释药系统重点研究室,南京210028

出　　处：《中国实验方剂学杂志》2013年第24期32-35,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省名医效方研究开发与评价业务建设项目(BM200903);江苏省中医药领军人才项目(LJ200913)

摘　　要：目的:优选贞栀颗粒中女贞子和白花蛇舌草的乙醇提取工艺。方法:采用HPLC测定特女贞苷、齐墩果酸和熊果酸含量。通过单因素试验考察影响特女贞苷转移率的因素,以特女贞苷和总三萜酸含量为评价指标,采用正交试验考察乙醇体积分数、乙醇用量、提取次数及提取时间对贞栀颗粒醇提工艺的影响。结果:最佳提取工艺为女贞子粗粉碎后与白花蛇舌草合并,分别加9,7倍量70%乙醇提取2次,每次1.5 h;特女贞苷、总三萜酸提取量分别为19.083,7.163 mg·g-1。结论:优选的提取工艺合理、稳定可行,为贞栀颗粒工业化生产提供参考。Objective： To optimize ethanol extraction technology of Ligustri Lucidi Fructus and Herba Hedyotis from Zhenzhi granules. Method： Contents of oleanolic acid,ursolic acid and specnuezhenide were determined by HPLC.Factors influencing transfer rate of specnuezhenide were investigated by single factor tests,with contents of specnuezhenide and total triterpenoid acids as indexes,orthogonal test was adopted to optimize ethanol extraction technology of Zhenzhi granules by taking the amount and concentration of ethanol,extraction time and times as factors. Result： Optimum extraction process was as following：coarse crushed Ligustri Lucidi Fructus and mixed with Herba Hedyotis,extracting twice with 70% ethanol （9 folds for the first time,7 folds for the second time） for 1.5 h each time;Extraction amount of specnuezhenide and total triterpenoid acids were 19.083 and 7.163 mg·g-1,respectively. Conclusion： This optimized extraction process was reasonable,stable and feasible,which could provide scientific basis for industrial production of Zhenzhi granules.

关 键 词：贞栀颗粒 女贞子 特女贞苷 齐墩果酸 熊果酸 正交试验 醇提工艺 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]