鸡血藤药材及其不同部位缩合鞣质的含量测定  

Determination of Condensed Tannins in Spatholobi Caulis

作　　者：林惠贞[1] 刘浩文[1] 韦玮[1] 周盈利[1] 陈建萍[2] 王冬梅[1] 

机构地区：[1]中山大学药学院,广州510006 [2]香港大学中医药学院

出　　处：《中国实验方剂学杂志》2013年第24期70-74,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技计划国际合作项目(2012B050300014);香港创新科技基金(ITF)(ITS/073/IIFP);2011年中山大学实验室开放基金项目(KF201142)

摘　　要：目的： 建立鸡血藤药材中缩合鞣质的含量测定方法，并对不同批次鸡血藤药材及其不同部位中缩合鞣质的含量进行测定。方法： 优化并建立了采用香草醛-硫酸显色体系测定鸡血藤药材中缩合鞣质含量的方法，反应条件为3 mL 3%香草醛，3 mL 40%浓硫酸，常温反应20 min，测定波长为500 nm。结果： 5个批次鸡血藤药材中缩合鞣质含量为3.99%-8.63%；其中3个批次药材不同部位的缩合鞣质含量测定结果显示，树脂状分泌物中含量最高，为8.14%-11.51%，木质部（5.39%-7.92%）和韧皮部（3.65%-6.90%）含量相对较少。结论： 香草醛-硫酸显色法快速、简便、准确、重复性好， 可用于测定鸡血藤药材中缩合鞣质的含量；缩合鞣质在树脂状分泌物中的含量比其他部位高，为传统的鸡血藤药材质量的判断方法提供了科学依据。Objective： To establish a method for determination of condensed tannins in Spatholobi Caulis （the stem of Suberect spatholobus） and investigate the content of condensed tannins in Spatholobi Caulis and its different parts. Method： The vanillin-sulphuric acid coloration system for the determination of condensed tannins was set up. The optimized condition of coloration was 3 mL 3% vanillin, 3 mL 40% sulphuric acid, at room temperature for 20 min, and the detection wavelength was set at 500 nm. Result： The content of condensed tannins was measured as 3.99%-8.63% in five commercial crude drugs of Spatholobi Caulis. Among different parts of the crude drugs, condensed tannins was most abundant in the resin-like secretion part （8.14%-11.51%）, far higher than those in the xylem part （5.39%-7.92%） and the phloem part （3.65%-6.90%）. Conclusion： The developed methods are simple, rapid, accurate, stable, and reproducible with high selectivity, and are suitable for the determination of condensed tannins in the quality control of Spatholobi Caulis.

关 键 词：鸡血藤 密花豆属密花豆 缩合鞣质 香草醛-硫酸显色法 质量控制 树脂状分泌物 

分 类 号：R284.1[医药卫生—中药学]