HPLC同时测定夏桑菊颗粒中迷迭香酸与异迷迭香酸苷的含量  

Simultaneous Determination of Rosmarinic Acid and Salviaflaside in Xiasangju Granules of by HPLC

作　　者：花汝凤 姚江雄 黎志坚 方铁铮 蒋丽娟 许招懂 

机构地区：[1]广州白云山星群(药业)股份有限公司,广州510288

出　　处：《中国实验方剂学杂志》2013年第24期75-77,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部重大新药创制项目(2011ZX09401-404-02)

摘　　要：目的： 建立夏桑菊颗粒中迷迭香酸、异迷迭香酸苷2成分含量测定方法。方法： 采用HPLC同时测定夏桑菊颗粒中迷迭香酸、异迷迭香酸含量，Agilent Eclipse XDB-C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.1%磷酸梯度洗脱，流速1.0 mL·min-1，检测波长319 nm，柱温30℃。结果： 迷迭香酸、异迷迭香酸苷线性范围分别为0.036 4-0.364 μg（R2=0.999 8），0.004 98-0.049 8 μg（R2=0.999 7）；迷迭香酸平均回收率为99.16%，RSD 1.32%，异迷迭香酸苷平均回收率为98.40%，RSD 0.78%（n=6）。结论： 该方法操作简单，可靠，重复性好，可用于控制夏桑菊颗粒质量控制。Objective： To develop a HPLC method for simultaneous determination of rosmarinic acid and salviaflaside in Xiasangju Granules. Method： An Agilent Eclipse XDB-C18 column （4.6 mm×250 mm, 5 μm） was applied with gradient elution using acetonitrile and 0.1% phosphoric acid as mobile phase, and the flow rate was 1.0 mL·min-1. The UV detector was set at 319 nm. Result： The linear range of rosmarinic acid was 0.036 4-0.364 μg, and the average recovery was 99.16%. The linear range of salviaflaside was 0.004 98-0.049 8 μg and the average recovery was 98.40%（n=6）. Conclusion： The method is accurate and repeatable. It can be used for quality evaluation of Xiasangju Granules.

关 键 词：夏桑菊颗粒 迷迭香酸 异迷迭香酸苷 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]