参芪益智组分中药的质量标准研究  

Study on Quality Standard of Monomer Combination Drug of Shenqi Yizhi

作　　者：周威[1] 雷永涛[1] 梁妍[1] 郝小燕[1] 

机构地区：[1]贵阳医学院药学院,贵阳550004

出　　处：《中国实验方剂学杂志》2013年第24期93-96,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金(81360681);贵州省中药现代化基金(黔科合中药字[2010]5005号);贵阳市科技局基金([2010]筑科农合同字第1-中06号);贵州省科学技术基金(黔科合丁字[2013]2052号

摘　　要：目的:建立参芪益智组分中药的质量标准,为其质量标准的制定提供参考。方法:根据不同成分采用不同的提取方法处理样品,改用不同的溶剂系统对其薄层鉴别方法进行研究。采用高效液相色谱法测定参芪益智组分中药中4种黄酮成分的含量。结果:建立了参芪益智组分中药中阿魏酸、天麻素、淫羊藿总黄酮、人参总皂苷、黄芪总皂苷的薄层鉴别方法。参芪益智组分中药中4种黄酮成分线性方程、灵敏度、精密度、稳定性等均符合高效液相色谱法含量测定要求。结论:所建立的方法简便、可行、灵敏、准确、专属性强,重复性好,可作为参芪益智组分中药的质量控制方法。Objective： To establish the quality standard of monomer combination drug of Shenqi Yizhi, which could be appliable to establishing the foundation of its quality standards. Method： Due to different kinds of components in the drug, different extraction methods for sample preparation was adopted herein, several developer systems were investigated in this TLC study. A HPLC method was established for the determination of four flavonoids of this monomer combination drug. Result： TLC indentification methods of ferulaic acid, gastrodine, epimedium total flavonoids, ginseng total saponins and astragalus total saponins in monomer combination drug of Shenqi Yizhi were finally established respectively. Linear equations, sensitivities, precisions, stabilities, etc of four flavonoids of this drug under the HPLC were all in line with requirements of HPLC assay. Conclusion： The reported methods are simple, practical, sensitive, accurate, specific and reproducible, which can be used as quality control method of monomer combination drug of Shenqi Yizhi.

关 键 词：组分中药 人参总皂苷 黄芪总皂苷 质量标准 薄层鉴别 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]