黔产盾叶唐松草叶挥发油GC-MS分析及生物活性研究  

GC-MS Analysis and Biological Activity of Essential Oil from Leaves of Thalictrum ichangense

作　　者：刘敏洁[1] 赵琦[1] 梁娜[1] 刘明川[1] 杨胜杰[1] 杨松[1] 

机构地区：[1]绿色农药与农业生物工程国家重点实验室培育基地,教育部绿色农药与农业生物工程重点实验室,贵州大学精细化工研究开发中心,贵阳550025

出　　处：《中国实验方剂学杂志》2013年第24期135-138,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(21172048);贵州省社会发展攻关计划项目(20103052)

摘　　要：目的:研究黔产盾叶唐松草Thalictrum ichangense叶挥发油的化学成分及其抗氧化和抗肿瘤的生物活性。方法:采用超临界CO2萃取法提取盾叶唐松草叶的挥发油,通过气相色谱-质谱联用技术(GC-MS)分析其化学成分,用色谱峰面积归一法计算各组分相对百分含量,并分别采用DPPH·法和MTT法研究其抗氧化和抗肿瘤的生物活性。结果:从超临界CO2萃取法提取得到的挥发油中鉴定了21个化学成分,已鉴定出的组分占挥发油总量的91.86%;在300 mg·L-1时,挥发油对DPPH·清除率为(61.2±2.1)%,对MGC-803胃癌细胞体外增殖抑制率为(56.0±6.8)%。结论:盾叶唐松草叶挥发油中主要化学成分为正四十四烷(Tetratetracontane,25.82%)、3,5-二烯豆甾烷(Stigmastan-3,5-diene,16.39%)、二十八烷(Octacosane,14.96%)等。盾叶唐松草叶挥发油具有较好的抗氧化活性和对MCG-803体外增殖的抑制作用。Objective： To study the chemical compositions of the essential oil from the leaves of Thalictrum ichangense. Method： The essential oil was extracted by supercritical carbon dioxide extraction assay, and the constituents were identified by gas chromatography-mass spectrometer （GC-MS）. The DPPH·method was used to study its antioxidant activity and MTT assay was used to study its anti-tumor activity. The relative content of each component was calculated by area normalization method. Result： Twenty one compounds were identified, representing 91.86% of the essential oil. The scavenging ratio for the DPPH·radicals reaches （61.2±2.1）% when the concentration was 300 mg·L-1 and the essential oil at 300 mg·L-1 showed anti-tumor activities against MGC-803 in vitro with a inhibition rate of （56.0±6.8）%. Conclusion： The principal chemical constituents of the essential oil were tetratetracontane （25.82%）, stigmastan-3,5-diene （16.39%）, and octacosane （14.96%）. The essential oil showed certain antioxidant activity and displayed good inhibition proliferation of MCG-803 in vitro. The analytic results can provide further exploitation and utilization of T. ichangense.

关 键 词：盾叶唐松草 超临界CO2萃取 挥发油 气相色谱·质谱联用技术 抗氧化 

分 类 号：R284.1[医药卫生—中药学] R285.5[医药卫生—中医学]