加味五子衍宗方抑制脂多糖诱导的BV-2小胶质细胞炎症反应及机制研究  

Inhibitory Effects of Modified Wuzi Yanzong Prescription on LPS-induced Inflammatory Response on BV-2 Microglial Cells

作　　者：宋芳娇[1] 宋宜祥[1] 曾克武[1,2] 屠鹏飞[2] 王学美[1] 

机构地区：[1]北京大学第一医院中西医结合研究室,北京100034 [2]北京大学医学部药学院,北京100191

出　　处：《中国实验方剂学杂志》2013年第24期177-180,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(81173369);北京市自然科学基金面上项目(7132210);教育部博士点基金(20120001110105)

摘　　要：目的:研究加味五子衍宗方(MWP)对脂多糖(LPS)诱导的BV-2小胶质细胞炎症反应的抑制作用及潜在机制。方法:体外培养BV-2小胶质细胞系,用LPS(1 mg·L-1)诱导炎症模型,将细胞分为空白对照组,炎症模型组和MWP药物组,药物组MWP设置50,100,200 mg·L-13个质量浓度。LPS和药物处理细胞后分别检测各组细胞炎症因子一氧化氮(NO)的表达、诱导型一氧化氮合成酶(iNOS),环氧合酶-2(COX-2)的表达、核转录因子-κB(NF-κB)核转位以及还原型辅酶Ⅱ(NADPH)氧化酶活性和活性氧(ROS)的表达。结果:MWP可剂量依赖性降低LPS激活的BV-2小胶质细胞内iNOS,COX-2的蛋白表达和NO的释放,抑制NF-κB的核转位,此外还可剂量依赖性降低NADPH氧化酶活性并明显降低ROS的产生。结论:MWP可有效抑制LPS诱导的BV-2小胶质细胞炎症反应,可能是通过抑制NF-κB信号通路介导的炎症反应以及NADPH氧化酶介导的氧化应激实现的。Objective： To study the inhibitory effects of modified Wuzi Yanzong prescription （MWP） on BV-2 microglial cells induced by lipopolysaccharides（LPS） and the potential mechanism. Method： BV-2 microglial cells were cultured in vitro and stimulated by LPS （1 mg·L-1）.The cells were divided into three groups：blank control group, model group and MWP treatment group. The gradient 50, 100, 200 mg·L-1three concentrations were used in the treatment group. The expression of inflammatory factor nitric oxide（NO）, protein inducible nitric oxide synthese（iNOS） and cyclooxygenase（COX-2）, the translocation of nuclear factor-κB （NF-κB）, nicotinamide adenosine denucleotide hydrophosphori（NADPH） oxidase activity and reactive oxygen species were tested after treating the cells by LPS and MWP. Result： MWP could decrease the release of NO and expression of iNOS, COX-2 protein with a dose-dependent manner, the nuclear translocation of NF-κB was inhibited by MWP too. In addition, MWP could decrease the NADPH oxidase activity with a dose-dependent manner and significantly reduced the generation of ROS. Conclusion： MWP can significantly inhibit the inflammatory response of BV-2 microglial cells induced by LPS via suppression of NF-κB signaling pathway and NADPH oxidase-mediated oxidative stress.

关 键 词：神经炎症 BV-2小胶质细胞系 加味五子衍宗方 抗炎 神经保护 

分 类 号：R285.5[医药卫生—中药学]