四妙勇安汤抑制中性粒细胞-内皮细胞黏附的实验研究  

Simiao Yongan Tang Inhibits Neutrophil-endothelial Cell Adhesion

作　　者：李光辉[1] 张军平[2] 丁义[1] 曹阳[1] 董玮[1] 彭立[1] 

机构地区：[1]天津中医药大学,天津300193 [2]天津中医药大学第一附属医院心血管内科,天津300193

出　　处：《中国实验方剂学杂志》2013年第24期193-196,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(901904);天津市自然科学基金重点项目(09JCZDJC20700)

摘　　要：目的:探讨四妙勇安汤对于凝血酶处理的内皮细胞与中性粒细胞黏附及内皮细胞P-选择素表达的影响。方法:MTT法检测四妙勇安汤以生药22.5 g·kg-1·d-1,ig 7 d制备含药血清对于内皮细胞活力的影响,EA.hy926细胞加入0.2 U·mL-1凝血酶、中性粒细胞作为模型组,2.5%,5%,10%,20%四妙勇安汤含药血清预处理内皮细胞24 h后造模为干预组。虎红法检测细胞黏附率,流式细胞术、ELISA检测内皮细胞P-选择素的表达。结果:空白组与空白对照组在各项检测中均无显著性差异;10%,20%含药血清组对黏附率有抑制作用,10%组效果较好;ELISA结果显示10%含药血清组预处理组能抑制P-选择素的表达,其他3个干预组则无差异;流式细胞术检测10%,20%含药血清组对于细胞表面P-选择素有抑制作用,10%含药血清组效果最好。结论:四妙勇安汤能抑制中性粒细胞、内皮细胞的黏附,这一作用可能是通过抑制内皮细胞P-选择素的表达来实现的。Objective： To investigate the impact of Simiao Yongan Tang on the expression of thrombin-treated endothelial cells and neutrophil adhesion and endothelial cell P-select expression. Method： MTT was used to detect endothelial cell viability of serum containing Simiao Yongan Tang 0.2 U·mL-1 thrombin was added to EA.hy926 cells,neutrophils was as a model group, 2.5%, 5%, 10%, 20% of the serum containing drugs pretreated endothelial cells 24 h after modeling.Bengal method was used to detect the cell adhesion rate, the flow cytometry and ELISA were used to detect endothelial cell P-selectin expression. Result： 10%, 20% serum containing drugs inhibited adhesion rate, 10% serum of which was better;the ELISA results showed that 10% serum containing drugs pretreatment could inhibited P-selectin expression;the flow cytometry showed that 10%, 20% serum containingdrugs inhibited cell surface P-selectin, 10% serum containing drugs was better. Conclusion： Simiao Yongan Tang can inhibit neutrophil adhesion of endothelial cells, this effect may be achieved by inhibiting P-selectin expression in endothelial cells.

关 键 词：四妙勇安汤 黏附 内皮细胞 中性粒细胞 P-选择素 

分 类 号：R285.5[医药卫生—中药学]