冠心舒胶囊对冠状动脉结扎大鼠心肌缺血的保护作用  

Protective Effects of Guanxinshu Capsule on Myocardial Ischemia Induced by Ligating Coronary Artery in Rats

作　　者：刘婷[1] 曹春雨[1] 回连强[1] 郝然[1] 王彦礼[1] 高双荣[1] 王海林[1] 臧琛[1] 陈艳军[1] 李春[1] 杨立新[1] 张毅[1] 周田明 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]鹰潭市艾瑞特生物医药科技有限公司,江西鹰潭335000

出　　处：《中国实验方剂学杂志》2013年第24期206-210,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(90709043)

摘　　要：目的:研究冠心舒胶囊对冠状动脉结扎所致大鼠心肌缺血的治疗作用。方法:以结扎左冠状动脉前降支方法建立大鼠心肌缺血模型,设正常对照组、假手术组、模型组、复方丹参滴丸90 mg·kg-1组(阳性药)、冠心舒低、高剂量组(生药量为5.2,15.6 g·kg-1),连续给药28 d。末次给药后,记录大鼠II导联心电图;TTC染色法测定心肌梗死范围;测定血浆中凝血酶时间(PT)、活化部分凝血激酶时间(APTT);血清中肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)和心肌组织中谷胱甘肽过氧化物酶(GSH-Px)活性;切取左心室进行心肌病理观察,并进行病理分级评分。结果:冠心舒能显著缩小心肌梗死范围(与模型组比较,P<0.05),改善大鼠心电图ST段的压低(P<0.05),显著降低CK,CK-MB,LDH活力(P<0.05),延长APTT(P<0.01),增加心肌组织中GSH-Px活性(P<0.01),并对冠状动脉结扎所致大鼠心肌细胞的病理改变具有一定程度的改善作用。结论:冠心舒对冠状动脉结扎所致大鼠心肌缺血具有明确的治疗作用。Objective： To observe the therapeutic effect of Guanxinshu capsule on myocardial ischemia induced by ligating coronary artery in rats. Method： Myocardial ischemia model was developed by ligating the anterior descendingbranch of left coronary artery in rats. The rats were randomly divided into normal control group, sham group, model group, Fufang Danshen pill 90 mg·kg-1 group（positive control）, Guanxinshu low and high dose group（raw materials 5.2,15.6 g·kg-1）,the rats were administered with medicine for 28 days. After the last administration, rat II lead electrocardiogram was recorded, the myocardial infarction was measured by triphenyltetrazolium chloride （TTC） staining method, prothrombin time（PT） and activated partial thromboplastin time（APTT） of plasma were detected, the activities of creatine kinase （CK）, isoenzyme of creatine kinase-MB（CK-MB）, lactate dehydrogenase（LDH） of serum and glutathione peroxidase（GSH-Px） in myocardial tissue were determined, the ventriculus sinister were dissected to assess the morphology by pathological grading. Result： Guanxinshu capsule could reduce the scope of myocardial infarct significantly（compared with sham group, there were significant difference, P〈0.05）, inhibit the lowering of ST segment （P〈0.05）, decrease the level of CK, CK-MB and LDH （P〈0.05）, prolong the time of APTT （P〈0.01）, increase the activity of GSH-Px （P〈0.01）, and improve the pathological changes of myocardial cells. Conclusion： Guanxinshu capsule showed obvious therapeutic effects on myocardial ischemia induced by ligating the coronary artery in rats.

关 键 词：冠心舒胶囊 冠状动脉结扎 心肌缺血 

分 类 号：R285.5[医药卫生—中药学]