3种重金属对欧洲花楸悬浮细胞生物量的影响  

Effect of Three Heavy Metals on Cell Suspension Cultures of Sorbus aucuparia Biomass

作　　者：黄蕾[1] 肖文娟[1] 刘超[1] 杨光[1] 郝庆秀[1] 吴志刚[1] 郭兰萍[1] 

机构地区：[1]中国中医科学院中药资源中心,北京100700

出　　处：《中国实验方剂学杂志》2013年第24期226-229,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81130070;81072989);国家科技支撑计划项目(2012BAI29B02);国家中医药管理局行业科研专项(201107009)

摘　　要：目的:以欧洲花楸悬浮培养细胞(SASC)为材料,研究非生物诱导子-重金属对SASC生物量的影响。方法:配制不同浓度的氯化镧、硝酸铜、硝酸铅溶液对SASC进行处理,用电子天平进行SASC生物量测定。结果:低浓度氯化镧刺激细胞生长而高浓度则抑制细胞生长,对细胞生长的影响表现为Hormesis现象,最适刺激质量浓度为0.01 mg·L-1;低浓度硝酸铜抑制细胞生长,高浓度刺激细胞生长,0.05 mg·L-1硝酸铜处理后细胞生物量最低;基本所有浓度的硝酸铅都表现为刺激细胞生长,最适刺激质量浓度为0.5 mg·L-1。结论:重金属诱导子影响了SASC的生物量,且其作用程度和方式与氯化镧、硝酸铜、硝酸铅3种诱导子种类及浓度相关。Objective： The cell suspension cultures of Sorbus aucuparia （SASC） was used to study the effect of abiotic elicitors-heavy metals on the SASC biomass. Method： The different concentrations of lanthanum chloride, copper nitrate, lead nitrate solution were prepared to treat the SASC,an electronic balance was used for measurement of Biomass of SASC. Result： The cell growth was stimulated by low concentration of lanthanum chloride but inhibited by high concentration of lanthanum chloride, the effect on cell growth was similar to Hormesis phenomenon, optimal stimulating concentration was 0.01 mg·L-1;low concentration of copper nitrate inhibited cell growth but high concentrations stimulated cell growth, after treated with 0.05 mg·L-1 copper nitrate the cell biomass was the lowest;substantially all concentrations of lead nitrate stimulated cell growth,the optimal stimulating concentration was 0.5 mg·L-1. Conclusion： Heavy metals affect the SASC biomass, and its role in the extent and manner may be related to elicitors＇ type and concentration.

关 键 词：重金属(镧铜铅) 欧洲花楸 生物量 

分 类 号：R285.5[医药卫生—中药学]