补阳还五汤对肺纤维化大鼠肺组织TGF-β_1/Smad3表达的影响  

Effects of Buyang Huanwu Decoction on Expression of TGF-β_1/Smad3 in Pulmonary Fibrosis Rats

作　　者：杨晗[1] 王飞[1] 王全林[1] 钟振东[2] 李斌[1] 杨昆[1] 

机构地区：[1]成都中医药大学,成都610075 [2]四川省人民医院实验动物研究所,成都610212

出　　处：《中国实验方剂学杂志》2013年第24期240-244,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国教育部博士点项目(20115132110002)

摘　　要：目的:观察补阳还五汤对博莱霉素致肺纤维化大鼠肺组织病理及转化生长因子β1(TGF-β1)m RNA,Smad3mRNA表达的影响。方法:将144只健康雄性SD大鼠随机分成6组,即假手术(NS)组、模型(BLM)组、强的松(P)组、补阳还五汤高、中、低(A,B,C)组,每组各24只。除NS组外,其余各组采用一次性气管滴注博莱霉素复制肺纤维化大鼠模型,NS组气管内滴注等量NS,造模第2天起,A,B,C组用补阳还五汤(剂量分别为18.48,9.24,4.62 g·kg-1)灌胃,P组用强的松混悬液(4.2 mg·kg-1)灌胃,NS组及BLM组用NS(10 mL·kg-1)灌胃,于造模后第7,14,28 d分3批处死动物,每次8只,取左肺制备病理切片,行HE,Masson染色观察肺泡炎及肺纤维化程度改变情况;取右肺上中叶组织,采用逆转录-聚合酶链反应(RTPCR)法检测各组大鼠肺组织TGF-β1mRNA,Smad3 mRNA的表达。结果:①BLM组大鼠肺泡炎及肺纤维化程度在不同时间点均明显高于同期NS组,差异明显(P<0.01);②与BLM组相比,补阳还五汤各剂量组肺泡炎及肺纤维化程度有不同程度的改善(P<0.01或P<0.05);③与NS组相比,BLM组肺组织TGF-β1,Smad3表达水平在不同时间点均明显增高(P<0.01),尤以第7天最为明显,并随时间推移有逐渐下降的趋势;④补阳还五汤各剂量组TGF-β1,Smad3表达水平与同期BLM组相有不同程度的降低(P<0.01或P<0.05),其中B组在7,14,28 d,C组在14,28 d下降较明显(P<0.01),A组与同期BLM组相比虽有所降低,但早期不如B,C组明显。结论:补阳还五汤可以明显改善模型大鼠肺组织肺泡炎和肺纤维化程度,抑制博莱霉素诱导的肺纤维化大鼠肺组织TGF-β1mRNA、Smad3mRNA的表达上调,从而减轻肺纤维化程度。Objective： To investigate the effects of Buyang Huanwu decoction on pathomorphology and the expression of transforming growth factor-β1（TGF-β1）mRNA and Smad3 mRNA in lung tissue of pulmonary fibrosis rats induced by bleomycin （BLM）. Method： 144 healthy male SD rats were randomly divided into six groups：sham operation（NS） group,model（BLM）group,prednisone（P） group,Buyang Huanwu decoction high-dose, middle-dose, low-dose（A, B, C） group, 24 rats in each group. Pulmonary fibrosis model was established by injection of BLM into tracheal intubation, NS group was intratracheally instilled with equal NS. The rats of group A, B, C were given Buyang Huanwu decoction at the dose of 18.48,9.24,4.62 g·kg-1, the rats of group P were given prednisone at the dose of 4.2 mg·kg-1, the rats of group NS and BLM were given NS at the dose of 10 mL·kg-1. 8 rats in each group were killed respectively on days 7, 14, 28 after intratracheal instillation. To measure alveolar catarrh and pulmonary fibrosis degrees in left lung dyed with HE and Masson, and TGF-β1mRNA, Smad3 mRNA in right lung tissue was assessed by RT-PCR. Result： ①Alveolar catarrh and pulmonary fibrosis degrees in group BLM were significantly higher than group NS（P〈0.01）;②The groups of Buyang Huanwu decoction could improve the alveolar catarrh and pulmonary fibrosis in varying degree by comparison with group BLM （P〈0.01 or P〈0.05）;③At all time points, TGF-β1, Smad3mRNA expression in group BLM was higher than that in group NS（P〈0.01）,on the 7th day the change was significant;④TGF-β1, Smad3 mRNA expression of the groups Buyang Huanwu decoction was lower than that in group BLM（P〈0.01 or p〈0.05）, TGF-β1, Smad3 mRNA expression of group B was lower than that in group BLM at all time points（P〈0.01）, the levels of that in group C were significant lower than group BLM＇s on the 14th day and the 28th day（P〈0.01）, the levels of group A＇s were lower than that in group BLM, but the differences were

关 键 词：肺纤维化 补阳还五汤 转化生长因子Β1 SMAD3 

分 类 号：R285.5[医药卫生—中药学]