MAGE-1与IL-18基因重组质粒转染的树突状细胞诱导体内抗肿瘤作用研究  

Study of anti-cancer effect induced by transfected dendritic cell vaccine of recombinant plasmid of MAGE-1 and IL-18 in vivo

作　　者：李首庆[1] 马寅芙[1] 杨广民[1] 

机构地区：[1]吉林省人民医院医学诊治实验中心,长春130021

出　　处：《中国免疫学杂志》2013年第11期1151-1154,1160,共5页Chinese Journal of Immunology

基　　金：吉林省科技发展计划项目(No.200905192;200705449)

摘　　要：目的:研究MAGE-1与IL-18基因重组质粒转染树突状细胞在体内诱导的抗肿瘤作用。方法:将重组质粒转入DC中构建DC疫苗,免疫肝癌荷瘤小鼠,观察被免疫小鼠的成瘤情况和生存时间。结果:DC疫苗免疫的荷瘤小鼠比对照组肿瘤生长受抑制,生存时间延长,其中共表达疫苗(pcmIL-18-MAGE)-DC组效果最佳(P<0.05),对MAGE(+)肝癌杀伤作用明显。结论:MAGE-1与IL-18基因重组质粒转染的树突状细胞疫苗在体内可产生抗肿瘤效果。Objective： To observe lethal effect on cancer by the DCs vaccine transfected with recombinant plasmid pcmIL-18-MAGE. Methods： Recombinant plasmids were transfected into DC cells,which were immuned to murine liver carcinoma,and observed the tumor regression and the survival period. Results： The tumor size was obviously regressed and the survival period was prolonged in the DC vaccine groups,and the（ pcmIL-18-MAGE）-DC group was the best（ P 0. 05）,especiously to the MAGE（ ＋） liver cancer. Conclusion： The anti-cancer effect is obviously of the transfected DC vaccine of recombinant plasmid of MAGE-1 and IL-18.

关 键 词：疫苗 肝癌 树突状细胞 MAGE-1 IL-18 

分 类 号：R392.11[医药卫生—免疫学]