HIV/AIDS患者CD4^+ CD25^+ Foxp^(3+) CD45RO^+记忆性调节性T细胞变化研究  

Changes of CD4^+ CD25^+ Foxp3^+ CD45RO^+ memory regulatory T cells in HIV/AIDS patients of China

作　　者：张子宁[1] 刘静[1] 张旻[1] 姜拥军[1] 王亚男[1] 尚红[1] 

机构地区：[1]中国医科大学附属第一医院检验科卫生部艾滋病免疫学重点实验室,沈阳110001

出　　处：《中国免疫学杂志》2013年第11期1168-1173,共6页Chinese Journal of Immunology

基　　金："十二五"国家科技重大专项课题资助项目(2012ZX10001-006)资助

摘　　要：目的:对辽宁、吉林和河南等地HIV/AIDS患者记忆性调节性CD4+CD25+Foxp3+CD45RO+T细胞水平及与疾病进展相关性进行研究,探讨记忆性调节性T细胞在HIV感染疾病进程中发挥的作用。方法:选取74名HIV感染者(缓慢进展组、无症状HIV感染组、艾滋病组、抗病毒治疗组)及15例健康对照,应用流式细胞仪检测CD4+CD25+Foxp3+CD45RO+记忆性调节性T细胞表达水平,分析其与CD4+T细胞数量、病毒载量、淋巴细胞活化及凋亡水平的相关性。结果:HIV感染缓慢进展组及抗病毒治疗组CD4+CD25+Foxp3+CD45RO+调节性T细胞水平[(2.19±1.07)%,(2.66±1.78)%]明显低于无症状HIV感染组(4.05±2.30)%,艾滋病组(5.85±2.46)%,P<0.05,无症状HIV感染组(4.05±2.30)%及健康对照(3.18±1.17)%明显低于艾滋病组(5.85±2.46)%,P<0.05。未治疗HIV/AIDS患者CD4+CD25+Foxp3+CD45RO+记忆性调节性T细胞百分率与CD4+T细胞显著负相关(r=-0.690,P<0.001),与病毒载量明显正相关(r=0.401,P=0.002),与CD4+HLA-DR+、CD8+HLA-DR+、CD8+CD38+细胞显著正相关(P<0.05),与CD4+CD95+、CD8+CD95+T细胞水平显著正相关(P<0.05)。结论:HIV感染者CD4+CD25+Foxp3+CD45RO+记忆性调节性T细胞百分率明显升高,与疾病进展及免疫损伤显著相关,是影响艾滋病疾病进展的重要因素之一。Objective：To quantify the alternations of CD4^＋ CD25^＋ Foxp^3＋ CD45RO^ memory regulatory T ceils of HIV infec- ted patients from Liaoning, Henan and Jifin Provinces and to investigate the role of memory regulatory T cells in disease progression of HIV infection. Methods： 74 HIV infected patients and 15 normal controls were enrolled and the frequency ofCD4^＋ CD25^＋ Foxp^3＋ CD45RO^ regulatory T cells was detected by flow-cytometry. To study the correlation between CD4^＋ CD25^＋ Foxp^3＋ CD45RO^ regula- tory T cells and disease progression, the absolute CIM ~ T ceils, viral load and activation, apoptosis of T cells. Results： We found that the frequencies of CIM ~ CD25 ＋ Foxp3 ＋ CIMSRO ＋ regulatory T ceils in slow progressors （2.19 ± 1.07 ）% and patients received anti retrovi,＇al therapy（ 2.66 ± 1.78 ）% were significantly lower than that in asymptomatic HIV infected patients （4.05± 2.30）% and AIDS patients （5.85 ± 2.46）%, P 〈 0.05. The frequency ofCD4^＋ CD25^＋ Foxp^3＋ CD45RO^ regulatory T cells was inversely related to CD4^＋ T ceils and related to viral loads, HLA-DR, CD95 expression on CIM, CD8 ＋ T cells （P 〈 0.05）. Conclusion： The frequency of CD4^＋ CD25^＋ Foxp^3＋ CD45RO^ memory regulatory T cells was significantly increased in HIV infection and closely related to disease progression and immune responses, which indicate that alternation of memory regulatory T cells may play important role in HIV disease progresston.

关 键 词：HIV-1 记忆性调节性T细胞 活化 

分 类 号：R392.11[医药卫生—免疫学]